https://scholars.lib.ntu.edu.tw/handle/123456789/413364
標題: | Extract of ganoderma formosanum mycelium as a highly potent tyrosinase inhibitor | 作者: | Hsu K.-D. Chen H.-J. Wang C.-S. Lum C.-C. Wu S.-P. Lin S.-P. KUAN-CHEN CHENG SHAU-PING LIN |
公開日期: | 2016 | 卷: | 6 | 來源出版物: | Scientific Reports | 摘要: | In this study, the inhibitory effect of Ganoderma formosanum mycelium extracts on tyrosinase, the central regulatory enzyme being responsible for cutaneous pigmentation, was investigated in both cell-free and cellular enzymatic systems, as well as in phenotype-based zebrafish model. Bioassay-guided purification indicated that the ethyl acetate fraction of G. fromosanum mycelium ethanolic extract (GFE-EA) demonstrated the highest inhibition toward cell-free tyrosinase (IC 50 = 118.26 ¡Ó 13.34 ppm). The secreted and intracellular melanin of B16-F10 cells were reduced by GFE-EA through suppression of tyrosinase activity (IC 50 = 102.27 ¡Ó 9.49 ppm) and its protein expression. Moreover, GFE-EA decreased surface pigmentation level of zebrafish via down-regulation of tyrosinase activity. Most of all, there is no significant difference in morphology and mortality between control and GFE-EA treated groups. Not only does GFE-EA exhibit similar depigmenting efficacy to kojic acid with lower dosage (approximately one-seventh of dose), but show less toxicity to zebrafish. It is worth noting that GFE-EA is extracted from mycelium, which subverts the general concept that mycelium lacks certain bioactivities possessed by fruit bodies. Altogether, it would appear that GFE-EA has great potential for application in the cosmetics industry. ? 2016 The Author(s). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413364 | ISSN: | 20452322 | DOI: | 10.1038/srep32854 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84987678645&doi=10.1038%2fsrep32854&partnerID=40&md5=56977f4d8c6d798f11ea2af8103280c8 |
顯示於: | 食品科技研究所 |
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