https://scholars.lib.ntu.edu.tw/handle/123456789/413419
標題: | Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability | 作者: | Yang W.-C. Hsu T.-C. Cheng K.-C. JE-RUEI LIU KUAN-CHEN CHENG |
關鍵字: | Lactobacillus reuteri;Lactonohydrolase;Probiotics;Zearalenone | 公開日期: | 2017 | 卷: | 16 | 期: | 1 | 來源出版物: | Microbial Cell Factories | 摘要: | Background: Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue. Results: In this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101. Conclusions: To the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli. ? 2017 The Author(s). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413419 | ISSN: | 14752859 | DOI: | 10.1186/s12934-017-0687-8 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018564546&doi=10.1186%2fs12934-017-0687-8&partnerID=40&md5=1c70d77a057a8265ad71cb09a68e92bf |
顯示於: | 生物科技研究所 |
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s12934-017-0687-8.pdf | 1.92 MB | Adobe PDF | 檢視/開啟 |
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