https://scholars.lib.ntu.edu.tw/handle/123456789/413659
標題: | Reversion of gold nanoparticle aggregates for the detection of Cu2+ and its application in immunoassays | 作者: | Chang C.-C. Lee C.-H. Wu T.-H. Chen C.-P. Chen C.-Y. Lin C.-W. |
公開日期: | 2017 | 出版社: | Royal Society of Chemistry | 卷: | 142 | 期: | 24 | 起(迄)頁: | 4684-4690 | 來源出版物: | Analyst | 摘要: | A high concentration of copper is a hazardous element to organisms and human health. Although various strategies have been reported for the sensitive detection of copper, a facile and rapid detection of aqueous copper has seldom been addressed to date. Here, we present an easy and accessible colorimetric method to detect Cu2+ using the redispersion of cysteamine-modified gold nanoparticles (CA-AuNPs). Initially, CA caused the aggregation of AuNPs due to the electrostatic interaction and aggregated AuNPs can be regenerated in basic medium. The subsequent addition of Cu2+ to the CA-AuNP dispersion could effectively trigger the aggregation of CA-AuNPs, resulting from the coordination reactivity between the deprotonated CA and Cu2+. This strategy resulted in a detection limit (LOD) of 1.52 μM in drinking water, which is below the U.S. Environmental Protection Agency permissible limit (20 μM). To demonstrate the broad application of CA-AuNPs, we further applied this method to plasmonic immunoassays based on the competitive interaction of Cu2+ between CA-AuNPs and enzymes. The LOD of the Down syndrome biomarker hyperglycosylated human chorionic gonadotropin (H-hCG) was 0.125 mIU mL-1, which is better than that of commercial immunoassays. Importantly, the determination of H-hCG in serum indicates its applicability for the measurement of real samples. Our assay agrees well with the current immunoassay systems and thus it can easily be expanded to a more common sensing platform for different types of biotargets by changing the corresponding antibodies. ? This journal is The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037671986&doi=10.1039%2fc7an01511a&partnerID=40&md5=a8b74b7aea8aae7c410e6a74d0e66023 https://scholars.lib.ntu.edu.tw/handle/123456789/413659 |
ISSN: | 32654 | DOI: | 10.1039/c7an01511a | SDG/關鍵字: | chorionic gonadotropin; copper; drinking water; gold; metal nanoparticle; adult; colorimetry; enzyme linked immunosorbent assay; female; genetic procedures; human; immunoassay; limit of detection; male; pregnancy; urinalysis; Adult; Biosensing Techniques; Chorionic Gonadotropin; Colorimetry; Copper; Drinking Water; Enzyme-Linked Immunosorbent Assay; Female; Gold; Humans; Immunoassay; Limit of Detection; Male; Metal Nanoparticles; Pregnancy; Urinalysis |
顯示於: | 醫學工程學研究所 |
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