https://scholars.lib.ntu.edu.tw/handle/123456789/414175
標題: | Molecular characterization of a rice metal tolerance protein, OsMTP1 | 作者: | Yuan L. Yang S. Liu B. Zhang M. Wu K. |
關鍵字: | Heavy metal;Metal tolerance protein;Rice | 公開日期: | 2012 | 卷: | 31 | 期: | 1 | 起(迄)頁: | 67-79 | 來源出版物: | Plant Cell Reports | 摘要: | Rice (Oryza sativa L. 'Nipponbare') cDNA subtractive suppression hybridization (SSH) libraries constructed using cadmium (Cd)-treated seedling roots were screened to isolate Cd-responsive genes. A cDNA clone, encoding the rice homolog of Metal Tolerance Protein (OsMTP1), was induced by Cd treatment. Plant MTPs belong to cation diffusion facilitator (CDF) protein family, which are widespread in bacteria, fungi, plants, and animals. OsMTP1 heterologous expression in yeast mutants showed that OsMTP1 was able to complement the mutant strains' hypersensitivity to Ni, Cd, and Zn, but not other metals including Co and Mn. OsMTP1 expression increased tolerance to Zn, Cd, and Ni in wild-type yeast BY4741 during the exponential growth phase. OsMTP1 fused to green fluorescent protein was localized in onion epidermal cell plasma membranes, consistent with an OsMTP1 function in heavy metal transporting. OsMTP1 dsRNAi mediated by transgenic assay in rice seedlings resulted in heavy metal sensitivity and changed the heavy metal accumulation in different organs of mature rice under low-concentration heavy metal stress. Taken together, our results show that OsMTP1 is a bivalent cation transporter localized in the cell membrane, which is necessary for efficient translocation of Zn, Cd and other heavy metals, and maintain ion homeostasis in plant. ? 2011 Springer-Verlag. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414175 | ISSN: | 07217714 | DOI: | 10.1007/s00299-011-1140-9 |
顯示於: | 植物科學研究所 |
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