https://scholars.lib.ntu.edu.tw/handle/123456789/414180
標題: | Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor | 作者: | Chu P. Chen H. Zhou Y. Li Y. Ding Y. Jiang L. Tsang E.W.T. Wu K. Huang S. |
關鍵字: | Annexin;Arabidopsis;Proteomics;Sacred lotus;Seed thermotolerance;Seed vigor | 公開日期: | 2012 | 卷: | 235 | 期: | 6 | 起(迄)頁: | 1271-1288 | 來源出版物: | Planta | 摘要: | Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced annexin, NnANN1, from the embryonic axes of sacred lotus (Nelumbo nucifera Gaertn.) using comparative proteomics approach. Moreover, the expression of NnANN1 increased considerably in response to high-temperature treatment. Quantitative real-time PCR (qRT-PCR) revealed that the transcripts of NnANN1 were detected predominantly during seed development and germination in sacred lotus, implicating a role for NnANN1 in plant seeds. Ectopic expression of NnANN1 in Arabidopsis resulted in enhanced tolerance to heat stress in transgenic seeds. In addition, compared to the wild-type seeds, transgenic seeds ectopically expressing NnANN1 exhibited improved resistance to accelerated aging treatment used for assessing seed vigor. Furthermore, transgenic seeds showed enhanced peroxidase activities, accompanied with reduced lipid peroxidation and reduced ROS release levels compared to the wild-type seeds. Taken together, these results indicate that NnANN1 plays an important role in seed thermotolerance and germination vigor. ? 2011 Springer-Verlag. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414180 | ISSN: | 00320935 | DOI: | 10.1007/s00425-011-1573-y |
顯示於: | 植物科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。