https://scholars.lib.ntu.edu.tw/handle/123456789/414199
標題: | Arabidopsis BREVIPEDICELLUS Interacts with the SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA to Regulate KNAT2 and KNAT6 Expression in Control of Inflorescence Architecture | 作者: | Zhao M. Yang S. Chen C.-Y. Li C. Shan W. Lu W. Cui Y. Liu X. Wu K. |
關鍵字: | Carotenoids; Flower colour; Orchid (Oncidium); Plant pigments; Tissue-specific promoter | 公開日期: | 2015 | 卷: | 11 | 期: | 3 | 來源出版物: | PLoS Genetics | 摘要: | BREVIPEDICELLUS (BP or KNAT1), a class-I KNOTTED1-like homeobox (KNOX) transcription factor in Arabidopsis thaliana, contributes to shaping the normal inflorescence architecture through negatively regulating other two class-I KNOX genes, KNAT2 and KNAT6. However, the molecular mechanism of BP-mediated transcription regulation remains unclear. In this study, we showed that BP directly interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA (BRM) both in vitro and in vivo. Loss-of-function BRM mutants displayed inflorescence architecture defects, with clustered inflorescences, horizontally orientated pedicels, and short pedicels and internodes, a phenotype similar to the bp mutants. Furthermore, the transcript levels of KNAT2 and KNAT6 were elevated in brm-3, bp-9 and brm-3 bp-9 double mutants. Increased histone H3 lysine 4 tri-methylation (H3K4me3) levels were detected in brm-3, bp-9 and brm-3 bp-9 double mutants. Moreover, BRM and BP co-target to KNAT2 and KNAT6 genes, and BP is required for the binding of BRM to KNAT2 and KNAT6. Taken together, our results indicate that BP interacts with the chromatin remodeling factor BRM to regulate the expression of KNAT2 and KNAT6 in control of inflorescence architecture. ? 2015 Zhao et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414199 | ISSN: | 15537390 | DOI: | 10.1371/journal.pgen.1005125 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926354873&doi=10.1371%2fjournal.pgen.1005125&partnerID=40&md5=e37909a45259c894c239165b92178c95 |
SDG/關鍵字: | BRM protein; histone H3; adenosine triphosphatase; Arabidopsis protein; BRM protein, Arabidopsis; chromatin; histone; homeodomain protein; KNAT1 protein, Arabidopsis; KNAT2 protein, Arabidopsis; KNAT6 protein, Arabidopsis; nonhistone protein; SWI-SNF-B chromatin-remodeling complex; transcription factor; Arabidopsis thaliana; Article; brevipedicellus gene; chromatin assembly and disassembly; controlled study; gene control; gene expression regulation; gene interaction; histone methylation; in vitro study; in vivo study; inflorescence; internode; KNAT2 gene; KNAT6 gene; nonhuman; phenotype; plant gene; protein binding; regulator gene; Arabidopsis; chromatin; genetics; metabolism; mutation; Arabidopsis; Arabidopsis thaliana; Adenosine Triphosphatases; Arabidopsis; Arabidopsis Proteins; Chromatin; Chromatin Assembly and Disassembly; Chromosomal Proteins, Non-Histone; Gene Expression Regulation, Plant; Histones; Homeodomain Proteins; Inflorescence; Mutation; Transcription Factors |
顯示於: | 植物科學研究所 |
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journal.pgen.1005125.PDF | 8.26 MB | Adobe PDF | 檢視/開啟 |
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