https://scholars.lib.ntu.edu.tw/handle/123456789/414802
標題: | Assessment of Photodynamic Inactivation against Periodontal Bacteria Mediated by a Chitosan Hydrogel in a 3D Gingival Model | 作者: | P. C. Peng C. M. Hsieh C. P. Chen T. Tsai C. T. Chen |
關鍵字: | Chitosan hydrogel; Periodontal disease; Periodontal disease chitosan hydrogel; Photodynamic inactivation; Photodynamic inactivation | 公開日期: | 2016 | 卷: | 17 | 期: | 11 | 起(迄)頁: | 13 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D gingival model resembling the oral periodontal pocket to culture the biofilms of Staphylococcus aureus (S. aureus), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), and Porphyromonas gingivalis (P. gingivalis). The PDI efficacy of chitosan hydrogel was examined against periodontal biofilms cultured in this 3D gingival model. We found that the PDI effectiveness was limited due to leaving some of the innermost bacteria alive at the non-illuminated site. Using this 3D gingival model, we further optimized PDI procedures with various adjustments of light energy and irradiation sites. The PDI efficacy of the chitosan hydrogel against periodontal biofilms can significantly improve via four sides of irradiation. In conclusion, this study not only showed the clinical applicability of this chitosan hydrogel but also the importance of the light irradiation pattern in performing PDI for periodontal disease. ? 2016 by the authors. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414802 | ISSN: | 1422 0067 | DOI: | 10.3390/ijms17111821 | SDG/關鍵字: | chitosan; hydroxypropylmethylcellulose; tolonium chloride; chitosan; hydrogel; hydroxypropylmethylcellulose; photosensitizing agent; tolonium chloride; adhesion; Aggregatibacter actinomycetemcomitans; antimicrobial activity; Article; biofilm; cell survival; controlled study; gingiva; hydrogel; illumination; irradiation; light; nonhuman; periodontal pocket; photodynamics; polymerization; Porphyromonas gingivalis; Staphylococcus aureus; anatomic model; Bacterial Infections; chemistry; drug effects; gingiva; Gingival Diseases; human; hydrogel; microbiology; photochemotherapy; physiology; procedures; radiation response; therapeutic use; treatment outcome; Aggregatibacter actinomycetemcomitans; Bacterial Infections; Biofilms; Chitosan; Dose-Response Relationship, Radiation; Gingiva; Gingival Diseases; Humans; Hydrogel; Hypromellose Derivatives; Light; Models, Anatomic; Periodontal Pocket; Photochemotherapy; Photosensitizing Agents; Porphyromonas gingivalis; Staphylococcus aureus; Tolonium Chloride; Treatment Outcome |
顯示於: | 生化科技學系 |
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