https://scholars.lib.ntu.edu.tw/handle/123456789/416024
標題: | Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets | 作者: | Thirumalraj, B. Dhenadhayalan, N. Chen, S.-M. Liu, Y.-J. Chen, T.-W. Liang, P.-H. Lin, K.-C. |
關鍵字: | Bioimaging; Energy transfer; Fluorescence; Gold nanoparticles; L-Cysteine; Reduced graphene oxide | 公開日期: | 2018 | 卷: | 259 | 起(迄)頁: | 339-346 | 來源出版物: | Sensors and Actuators, B: Chemical | 摘要: | A highly sensitive and selective fluorogenic sensing of L-Cysteine (L-Cys) was implemented based on gelatin stabilized gold nanoparticles decorated reduced graphene oxide (rGO/Au) nanohybrid. The rGO/Au nanohybrid was prepared by the one-pot hydrothermal method and well characterized by different physiochemical techniques. The nanohybrid exhibits a weak fluorescence of rGO due to the energy transfer from the rGO to Au NPs. The rGO/Au nanohybrid shows enhanced fluorescence activity due to the restoration of quenched fluorescence of rGO/Au nanohybrid in presence of L-Cys. The rGO/Au nanohybrid exhibits much lower detection limit of 0.51 nM for L-Cys with higher selectivity. The fluorescence sensing mechanism arose from the fluorescence recovery due to the stronger interaction between Au NPs and L-Cys, and consequently, the energy transfer was prevented between rGO and Au NPs. The practicability of rGO/Au sensor was implemented by invitro bioimaging measurements in Colo-205 (colorectal adenocarcinoma) and MKN-45 (gastric carcinoma) cancer live cells with excellent biocompatibility. ? 2017 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85038079837&doi=10.1016%2fj.snb.2017.12.028&partnerID=40&md5=d7e120cfefab80b95b1c4b6aae599be1 https://scholars.lib.ntu.edu.tw/handle/123456789/416024 |
DOI: | 10.1016/j.snb.2017.12.028 | SDG/關鍵字: | Amino acids; Biocompatibility; Energy transfer; Fiber optic sensors; Fluorescence; Gold; Graphene; Metal nanoparticles; Nanoparticles; Bio-imaging; Colorectal adenocarcinoma; Enhanced fluorescence; Gold Nanoparticles; L-cysteine; Lower detection limit; Physiochemical techniques; Reduced graphene oxides; Nanostructured materials |
顯示於: | 化學系 |
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