https://scholars.lib.ntu.edu.tw/handle/123456789/416026
標題: | Aptamer-based fluorogenic sensing of interferon-gamma probed with ReS2 and TiS2 nanosheets | 作者: | Dhenadhayalan, N. Sriram, M.I. Lin, K.-C. |
關鍵字: | 2D nanosheets; Aptamer; Biosensor; Fluorescence; Interferon-gamma | 公開日期: | 2018 | 卷: | 258 | 起(迄)頁: | 929-936 | 來源出版物: | Sensors and Actuators, B: Chemical | 摘要: | The fluorogenic aptamer sensing of interferon-gamma (IFN-γ) was scrutinized using two-dimensional (2D) ReS2 and TiS2 nanosheets (NSs) as a platform. The IFN-γ an important cytokine, functions as a bio-indicator to detect infectious diseases such as tuberculosis and human immunodeficiency virus. This 2D NSs based aptamer sensor was implemented to induce the fluorescence off/on resulting from an aptamer, in the absence or presence of a target to be probed. The fluorescence emitting from the aptamer is quenched by interacting with NSs, while the ensuing fluorescence is recovered upon addition of target. Such a fluorescence off/on mechanism was proposed based on the behavior of fluorescence resonance energy transfer (FRET) between the aptamer and NSs. The fluorescence response exhibits linearity as a function of target, and the detection limit of IFN-γ was evaluated to be 57.6 and 82.7 pM for ReS2 and TiS2 NSs, respectively, being comparable to or even better than those methods adopted for probing IFN-γ. The selectivity property was also characterized with various targets, exhibiting a very specific selectivity for IFN-γ. The findings reveal that the aptamer-transition metal dichalcogenides (TMD) NSs will be a great sensing pair to the development of aptamer-based biosensors. Moreover, the biocompatibility and sensing capability of IFN-γ was implemented in human embryonic kidney 293T (HEK) live cells. This is the first report to emerging fluorogenic sensing of IFN-γ aptamer with 2D TMD, showing a promising trend for future design of biosensors. ? 2017 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85036647797&doi=10.1016%2fj.snb.2017.11.178&partnerID=40&md5=d46150b3d1fe9172a9607b8856b334ae https://scholars.lib.ntu.edu.tw/handle/123456789/416026 |
DOI: | 10.1016/j.snb.2017.11.178 | SDG/關鍵字: | Antibiotics; Biocompatibility; Biosensors; Diseases; Energy transfer; Fluorescence; Glycoproteins; Nanosheets; Titanium compounds; Transition metals; Viruses; Aptamers; Fluorescence resonance energy transfer; Human embryonic kidneys; Human immunodeficiency virus; Interferon-gamma; Specific selectivity; Transition metal dichalcogenides (TMD); Two Dimensional (2 D); Rhenium compounds |
顯示於: | 化學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。