https://scholars.lib.ntu.edu.tw/handle/123456789/416476
標題: | Electro-Engineered Polymeric Films for the Development of Sensitive Aptasensors for Prostate Cancer Marker Detection | 作者: | LIN-CHI CHEN P. Jolly A. Miodek D. K. Yang L. C. Chen M. D. Lloyd P. Estrela |
關鍵字: | AMACR (P504S); amperometric; aptamer; aptasensor; electrochemical; PEG; polypyrrole; prostate cancer | 公開日期: | 2016 | 起(迄)頁: | 1308-13141 | 來源出版物: | Acs Sensors | 摘要: | We report the development of a simple surface chemistry strategy for the construction of sensitive aptasensors on a polypyrrole (PPy)-polyethylene glycol (PEG) platform in order to provide enhanced antifouling properties. We report the covalent modification of a PPy film formed on a gold electrode by PEG molecules, without prior chemical functionalization of the pyrrole monomer. This process was mediated by electro-oxidation of amine groups present on the one of the PEG end chains. Polyhistidine modified aptamers were immobilized to this surface via a Nα,Nα-Bis(carboxymethyl)-l-lysine ANTA/Cu2+ redox complex covalently attached to the PPy-PEG adduct. The fabricated aptasensor was then utilized for the detection of α-methylacyl-CoA racemase (AMACR; P504S), an emerging biomarker for prostate cancer. Protein/aptamer interactions were monitored through variation of the copper redox signal, using the square wave voltammetry (SWV) technique. We demonstrate that the PPy-PEG-ANTA/Cu2+ hybrid material is characterized by enhanced antifouling properties and sensitivity. The aptasensor was able to detect AMACR down to 5 fM in both buffer and spiked human plasma with a limit of detection (LOD) of 0.15 fM and 1.4 fM, respectively. The developed aptasensor can be generalized for use with any type of aptamer-based sensor. ? 2017 American Chemical Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/416476 | DOI: | 10.1021/acssensors.6b00443 | SDG/關鍵字: | Amino acids; Chemical modification; Diseases; Electrooxidation; Hybrid materials; Polyethylene glycols; Surface chemistry; Urology; Voltammetry; AMACR (P504S); Amperometric; Aptamers; aptasensor; electrochemical; Prostate cancers; Polypyrroles |
顯示於: | 生物機電工程學系 |
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