https://scholars.lib.ntu.edu.tw/handle/123456789/416493
標題: | Different intestinal tropism of the G2b Taiwan porcine epidemic diarrhea virus-Pintung 52 strain in conventional 7-day-old piglets. | 作者: | CHIAN-REN JENG Peng, J.-Y. Chang, C.-Y. Kao, C.-F. Chang, Y.-C. Hsueh, C.-S. Jeng, C.-R. Cheng, I.-C. Pang, V. F. Tsai, P.-S. IVAN-CHEN CHENG PEI-SHIUE TSAI YEN-CHEN CHANG HUI-WEN CHANG VICTOR FEI PANG |
公開日期: | 2018 | 卷: | 237 | 起(迄)頁: | 69-75 | 來源出版物: | Veterinary Journal | 摘要: | The group 2b (G2b) porcine epidemic diarrhea virus (PEDV) that emerged in 2013 has since caused devastating diseases and economic loss. The full-length genome of the G2b Taiwan PEDV-Pintung 52 (PEDV-PT) strain and its intestinal tropism by evaluating the pathological changes in the original PEDV-PT infected field piglet and orally inoculation of either 10, 103, or 105 50% tissue culture infective dose/mL (TCID50/mL) of the plaque-purified PEDV-PT-Passage 5 (P5) in 7-day-old conventional piglets were analyzed. Phylogenetic analysis of the full-length genome indicated that the G2b Taiwan PEDV-PT strain was closely related to the North American G2b PEDV strains. Some pathological features of the G2b Taiwan PEDV-PT infection, including the absence of lesions and antigen signal in the crypt epithelial cells of the jejunum and ileum and in the villus enterocytes of the duodenum and colon, were different from those of infections by the North American G2b PEDV strains. This difference in the intestinal tropism of the G2b Taiwan PEDV-PT strain highlights the importance of studying the pathogenicities of different PEDV variants. Moreover, similar distributions of PEDV antigens and lesions in the G2b Taiwan PEDV-PT infected field piglet and its plaque-purified isolate, PEDV-PT-P5, inoculated piglets indicating that the plaque-purified PEDV-PT-P5 viral stock could facilitate the preclinical evaluation of vaccines and other interventions aimed at preventing the G2b PEDV infection. ? 2018 Elsevier Ltd |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/416493 | ISSN: | 1532-2971 1090-0233 | DOI: | 10.1016/j.tvjl.2018.06.002 | SDG/關鍵字: | animal experiment; animal model; animal tissue; Article; colon; controlled study; duodenum; histopathology; ileum; immunohistochemistry; intestine crypt; intestine epithelium cell; intestine villus; jejunum; molecular phylogeny; nonhuman; piglet; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; Taiwan; viral tropism; virus genome; virus shedding; virus virulence; animal; classification; Coronavirus infection; pathology; phylogeny; physiology; pig; Porcine epidemic diarrhea virus; swine disease; tropism; veterinary medicine; viral tropism; virology; Animals; Coronavirus Infections; Phylogeny; Porcine epidemic diarrhea virus; Swine; Swine Diseases; Taiwan; Tropism; Viral Tropism |
顯示於: | 獸醫學系 |
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