https://scholars.lib.ntu.edu.tw/handle/123456789/416946
標題: | Nuclear receptor interaction protein, a coactivator of androgen receptors (AR), is regulated by AR and Sp1 to feed forward and activate its own gene expression through AR protein stability | 作者: | Chen P.-H. Tsao Y.-P. Wang C.-C. SHOW-LI CHEN |
公開日期: | 2008 | 卷: | 36 | 期: | 1 | 起(迄)頁: | 51-66 | 來源出版物: | Nucleic Acids Research | 摘要: | Previously, we found a novel gene, nuclear receptor interaction protein (NRIP), a transcription cofactor that can enhance an AR-driven PSA promoter activity in a ligand-dependent manner in prostate cancer cells. Here, we investigated NRIP regulation. We cloned a 413-bp fragment from the transcription initiation site of the NRIP gene that had strong promoter activity, was TATA-less and GC-rich, and, based on DNA sequences, contained one androgen response element (ARE) and three Sp1-binding sites (Sp1-1, Sp1-2, Sp1-3). Transient promoter luciferase assays, chromatin immunoprecipitation and small RNA interference analyses mapped ARE and Sp1-2-binding sites involved in NRIP promoter activation, implying that NRIP is a target gene for AR or Sp1. AR associates with the NRIP promoter through ARE and indirectly through Sp1-binding site via AR-Sp1 complex formation. Thus both ARE and Sp1-binding site within the NRIP promoter can respond to androgen induction. More intriguingly, NRIP plays a feed-forward role enhancing AR-driven NRIP promoter activity via NRIP forming a complex with AR to protect AR protein from proteasome degradation. This is the first demonstration that NRIP is a novel AR-target gene and that NRIP expression feeds forward and activates its own expression through AR protein stability. ? 2007 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-38349138514&doi=10.1093%2fnar%2fgkm942&partnerID=40&md5=97c4740135e3370b98d0f66d37c732de https://scholars.lib.ntu.edu.tw/handle/123456789/416946 |
ISSN: | 0305-1048 | DOI: | 10.1093/nar/gkm942 | SDG/關鍵字: | androgen receptor; cytosine; DNA; guanine; luciferase; nuclear receptor interaction protein; prostate specific antigen; proteasome; transcription factor; transcription factor Sp1; unclassified drug; androgen responsive element; article; binding site; chromatin immunoprecipitation; complex formation; controlled study; DNA sequence; GC rich sequence; gene expression regulation; gene targeting; hormone responsive element; human; human cell; molecular cloning; nucleotide sequence; priority journal; promoter region; prostate cancer; protein degradation; protein stability; regulatory mechanism; RNA interference; TATA box; transcription initiation site; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Cell Line; Cell Line, Tumor; Humans; Male; Molecular Sequence Data; Nuclear Proteins; Promoter Regions (Genetics); Prostatic Neoplasms; Receptors, Androgen; RNA, Messenger; Sp1 Transcription Factor; Trans-Activation (Genetics); Up-Regulation |
顯示於: | 微生物學科所 |
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