https://scholars.lib.ntu.edu.tw/handle/123456789/416948
標題: | Inhibition of cell motility by troglitazone in human ovarian carcinoma cell line | 作者: | Yang Y.-C. Ho T.-C. SHOW-LI CHEN Lai H.-Y. Wu J.-W. Tsao Y.-P. |
公開日期: | 2007 | 卷: | 7 | 來源出版物: | BMC Cancer | 摘要: | Background: Troglitazone (TGZ) is a potential anticancer agent. Little is known about the effect of this agent on cancer cell migration. Methods: Human ovarian carcinoma cell line, ES-2 cells were treated with various concentrations of TGZ. Cell migration was evaluated by wound-healing and Boyden chamber transwell experiments. PPARγ expression was blocked by PPARγ small interfering RNA. The effects of TGZ on phosphorylation of FAK, PTEN, Akt were assessed by immunoblotting using phospho-specific antibodies. The cellular distribution of paxillin, vinculin, stress fiber and PTEN was assessed by immunocytochemistry. Results: TGZ dose- and time-dependently impaired cell migration through a PPARγ independent manner. TGZ treatment impaired cell spreading, stress fiber formation, tyrosine phosphorylation of focal adhesion kinase (FAK), and focal adhesion assembly in cells grown on fibronectin substratum. TGZ also dose- and time-dependently suppressed FAK autophosphorylation and phosphorylation of the C-terminal of PTEN (a phosphatase). At concentration higher than 10 μM, TGZ caused accumulation of PTEN in plasma membrane, a sign of PTEN activation. Conclusion: These results indicate that TGZ can suppress cultured ES-2 cells migration. Our data suggest that the anti-migration potential of TGZ involves in regulations of FAK and PTEN activity. ? 2007 Yang et al; licensee BioMed Central Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-41649101085&doi=10.1186%2f1471-2407-7-216&partnerID=40&md5=ed49eacb8a076c6f2944362c176c011b https://scholars.lib.ntu.edu.tw/handle/123456789/416948 |
ISSN: | 1471-2407 | DOI: | 10.1186/1471-2407-7-216 | SDG/關鍵字: | fibronectin; focal adhesion kinase; paxillin; peroxisome proliferator activated receptor gamma; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; protein kinase B; small interfering RNA; troglitazone; vinculin; 2,4 thiazolidinedione derivative; chroman derivative; troglitazone; antineoplastic activity; article; autophosphorylation; cancer cell culture; cancer growth; cancer inhibition; carboxy terminal sequence; cell membrane; cell migration; cell motility; cell spreading; cellular distribution; controlled study; dose time effect relation; enzyme activation; enzyme activity; enzyme phosphorylation; enzyme regulation; focal adhesion; human; human cell; immunoblotting; immunocytochemistry; ovary carcinoma; protein expression; protein localization; stress fiber; cell motion; comparative study; dose response; drug effect; female; ovary tumor; pathology; physiology; tumor cell line; Cell Line, Tumor; Cell Movement; Chromans; Dose-Response Relationship, Drug; Female; Humans; Ovarian Neoplasms; Thiazolidinediones |
顯示於: | 微生物學科所 |
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