https://scholars.lib.ntu.edu.tw/handle/123456789/417092
標題: | SV40 T/t-common polypeptide inhibits angiogenesis and growth of HER2-overexpressing human ovarian cancer | 作者: | Hsueh S.-P. Hsu W.-B. Wen C.-C. WON-BO WANG |
關鍵字: | angiogenesis; HER2; ovarian cancer; SV40 T/t-common; TSP-1; VEGF-A | 公開日期: | 2011 | 卷: | 18 | 期: | 12 | 起(迄)頁: | 859-870 | 來源出版物: | Cancer Gene Therapy | 摘要: | Human epidermal growth factor receptor 2 (HER2) is frequently overexpressed in human ovarian cancers and its overexpression is associated with increased angiogenesis, increased metastasis and reduced survival. Inhibition of HER2 in HER2-overexpressing cancers can lead to reduced angiogenesis and improved survival. Previously, we reported that SV40 T/t-common polypeptide has transcriptional repression activity and can inhibit HER2 expression. In this study, we investigated the effect of T/t-common on the angiogenesis-inducing activity of HER2-overexpressing human SK-OV-3 ovarian cancer cells. We found that compared to conditioned medium from control SK-OV-3 cancer cells, conditioned medium from T/t-common-expressing SK-OV-3 cells had a reduced ability to induce endothelial cell migration and tube formation in vitro and microvessel formation in vivo. These data indicate that T/t-common can inhibit the ability of SK-OV-3 cancer cells to induce angiogenesis. T/t-common was found to be able to downregulate the expression of several proangiogenic factors, including vascular endothelial growth factor-A, interleukin-8, basic fibroblast growth factor, matrix metalloproteinase-2 and urokinase-type plasminogen activator, and upregulate antiangiogenic factors, including thrombospondin-1 and tissue inhibitor of metalloproteinases-1 in SK-OV-3 cancer cells. Finally, we demonstrated that T/t-common could inhibit the angiogenesis and growth of HER2-overexpressing human ovarian tumor in NOD/SCID mice. Taken together, the data suggest that T/t-common had the potential to be developed as a new antiangiogenic agent specific for treating HER2-overexpressing ovarian cancers. ? 2011 Nature America, Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-81155130927&doi=10.1038%2fcgt.2011.55&partnerID=40&md5=17af6fe5888e0ba370993859c8cd8cd9 https://scholars.lib.ntu.edu.tw/handle/123456789/417092 |
ISSN: | 0929-1903 | DOI: | 10.1038/cgt.2011.55 | SDG/關鍵字: | antineoplastic agent; epidermal growth factor receptor 2; fibroblast growth factor 2; gelatinase A; interleukin 8; plasminogen activator; sv40 T t common polypeptide; thrombospondin 1; tissue inhibitor of metalloproteinase 1; unclassified drug; vasculotropin A; Adenovirus; angiogenesis; animal experiment; animal model; animal tissue; article; cancer cell; cancer inhibition; cell migration; controlled study; endothelium cell; female; gene overexpression; human; human cell; in vitro study; in vivo study; mouse; nonhuman; ovary cancer; priority journal; upregulation; Animals; Antigens, Polyomavirus Transforming; Cell Line; Cell Line, Tumor; Cell Movement; Cell Proliferation; Culture Media, Conditioned; Female; Gene Expression Regulation, Neoplastic; Humans; Mice; Mice, Inbred NOD; Neovascularization, Pathologic; Ovarian Neoplasms; Peptides; Receptor, erbB-2; Mus |
顯示於: | 微生物學科所 |
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