https://scholars.lib.ntu.edu.tw/handle/123456789/417119
標題: | Application of a fluorescent marker with quantitative bioburden methods to assess cleanliness | 作者: | HAO-YUAN CHANG Hung, I.-C. ARISTINE CHENG Chen A.-C. Chen, A.-C. Chen M.-W. YEUR-HUR LAI WANG-HUEI SHENG Sheng, W.-H. |
公開日期: | 2018 | 卷: | 39 | 期: | 11 | 起(迄)頁: | 1296-1300 | 來源出版物: | Infection Control and Hospital Epidemiology | 摘要: | Background Improvement of environmental cleaning in hospitals has been shown to decrease in-hospital cross transmission of pathogens. Several objective methods, including aerobic colony counts (ACCs), the adenosine triphosphate (ATP) bioluminescence assay, and the fluorescent marker method have been developed to assess cleanliness. However, the standard interpretation of cleanliness using the fluorescent marker method remains uncertain.Objective To assess the fluorescent marker method as a tool for determining the effectiveness of hospital cleaning.Design A prospective survey study.Setting An academic medical center.Methods The same 10 high-touch surfaces were tested after each terminal cleaning using (1) the fluorescent marker method, (2) the ATP assay, and (3) the ACC method. Using the fluorescent marker method under study, surfaces were classified as totally clean, partially clean, or not clean. The ACC method was used as the standard for comparison.Results According to the fluorescent marker method, of the 830 high-touch surfaces, 321 surfaces (38.7%) were totally clean (TC group), 84 surfaces (10.1%) were partially clean (PC group), and 425 surfaces (51.2%) were not clean (NC group). The TC group had significantly lower ATP and ACC values (mean ± SD, 428.7 ± 1,180.0 relative light units [RLU] and 15.6 ± 77.3 colony forming units [CFU]/100 cm2) than the PC group (1,386.8 ± 2,434.0 RLU and 34.9 ± 87.2 CFU/100 cm2) and the NC group (1,132.9 ± 2,976.1 RLU and 46.8 ± 119.2 CFU/100 cm2).Conclusions The fluorescent marker method provided a simple, reliable, and real-time assessment of environmental cleaning in hospitals. Our results indicate that only a surface determined to be totally clean using the fluorescent marker method could be considered clean. ? 2018 by The Society for Healthcare Epidemiology of America. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053722418&doi=10.1017%2fice.2018.222&partnerID=40&md5=21913897800837711442f011ea0d8bb3 https://scholars.lib.ntu.edu.tw/handle/123456789/417119 |
DOI: | 10.1017/ice.2018.222 | SDG/關鍵字: | adenosine triphosphate; fluorescent dye; bacterial count; comparative study; cross infection; health care facility; hospital service; human; infection control; luminescence; procedures; prospective study; sanitation; Taiwan; university hospital; Academic Medical Centers; Adenosine Triphosphate; Colony Count, Microbial; Cross Infection; Fluorescent Dyes; Housekeeping, Hospital; Humans; Infection Control; Luminescent Measurements; Patients' Rooms; Prospective Studies; Sanitation; Taiwan |
顯示於: | 護理學系所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。