https://scholars.lib.ntu.edu.tw/handle/123456789/417157
標題: | Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation | 作者: | Lin L.-L. Huang H.-C. Ogihara S. JIN-TOWN WANG Wu M.-C. McNeil P.L. CHIUNG-NIEN CHEN HSUEH-FEN JUAN |
關鍵字: | Annexin A4; Cell proliferation; Helicobacter pylori; Plasma membrane repair | 公開日期: | 2012 | 出版社: | MDPI AG | 卷: | 13 | 期: | 8 | 起(迄)頁: | 10176-10192 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Helicobacter pylori (H. pylori), the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that H. pylori also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA) have been considered to be major H. pylori virulence factors. Gastric cancer cells were infected with H. pylori wild type (vacA+/cagA+), single mutant (ΔvacA or ΔcagA) or double mutant (ΔvacA/ΔcagA) strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. OPEN ACCESS H. pylori disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca2+ influx. Ca2+-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to H. pylori exposure, also promote host cell proliferation. We propose that H. pylori activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of H. pylori-induced gastric carcinogenesis. ? 2012 by the authors; licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874058602&doi=10.3390%2fijms130810176&partnerID=40&md5=335399078d9d259393308a4525fddd85 https://scholars.lib.ntu.edu.tw/handle/123456789/417157 |
ISSN: | 1661-6596 | DOI: | 10.3390/ijms130810176 | SDG/關鍵字: | calcium ion; lipocortin 1; lipocortin 4; lysosome associated membrane protein 2; bacterial antigen; bacterial protein; cagA protein, Helicobacter pylori; calcium; messenger RNA; VacA protein, Helicobacter pylori; article; calcium transport; cancer cell culture; carcinogenesis; cell damage; cell membrane; cell proliferation; cell regeneration; controlled study; exposure; Helicobacter infection; Helicobacter pylori; host cell; human; human cell; nonhuman; protein expression; stomach cancer; adenocarcinoma; cell membrane; flow cytometry; genetics; Helicobacter pylori; metabolism; microbiology; pathogenicity; pathology; real time polymerase chain reaction; reverse transcription polymerase chain reaction; stomach tumor; tumor cell culture; Western blotting; Helicobacter pylori; Adenocarcinoma; Antigens, Bacterial; Bacterial Proteins; Blotting, Western; Calcium; Cell Membrane; Cell Proliferation; Flow Cytometry; Helicobacter Infections; Helicobacter pylori; Humans; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Stomach Neoplasms; Tumor Cells, Cultured |
顯示於: | 微生物學科所 |
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