https://scholars.lib.ntu.edu.tw/handle/123456789/417216
標題: | Isolation of a Helicobacter pylori protein, FldA associated with mucosa- associated lymphoid tissue lymphoma of the stomach | 作者: | Chang C.-S. Chen L.-T. JYH-CHIN YANG Lin J.-T. Chang K.-C. Wang, Jin-Town |
公開日期: | 1999 | 出版社: | W.B. Saunders | 卷: | 117 | 期: | 1 | 起(迄)頁: | 82-88 | 來源出版物: | Gastroenterology | 摘要: | Background and Aims: The growth of gastric mucosa-associated lymphoid tissue lymphoma (MALToma) seems to depend on the stimulation of Helicobacter pylori. We attempted to identify specific antigen(s) from H. pylori strains associated with MALToma. Methods: Membranous and secreted proteins of H. pylori were compared on sodium dodecyl sulfate-polyacrylamide gel electrophoresis by Western blot using sera from patients with MALToma. Results: A 19-kilodalton protein was seen in all strains isolated from patients with MALToma but uncommonly in other strains. The protein was purified and sequenced. Amino acid sequence comparison showed it was an FIdA homologue, a putative flavodoxin protein. DNA sequencing in 26 strains revealed that a nucleotide G insertion at position 481 of the fldA gene was more frequently observed in strains associated with MALToma than other strains (9/9 vs. 6/17; P = 0.002). The mutation caused a short truncation. A recombinant protein with this truncation was expressed and tested. Sera of 12 (70.6%) of 17 patients with MALToma were positive for the antibody to the recombinant protein, and 7 (16.7%) of 42 control patients were positive (12/17 vs. 7/42; P < 0.0001). Conclusions: Truncated FldA of H. pylori is associated with gastric MALToma. It may be involved in the pathogenesis of gastric MALToma. Antibody to this antigen could be used as a serological marker of the disease. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033029543&doi=10.1016%2fS0016-5085%2899%2970553-6&partnerID=40&md5=47ee8bbccbc10ac51fe191a1eed4ef1e https://scholars.lib.ntu.edu.tw/handle/123456789/417216 |
ISSN: | 0016-5085 | DOI: | 10.1016/S0016-5085(99)70553-6 | SDG/關鍵字: | flavodoxin; protein antibody; adult; aged; antibody response; article; bacterial gene; clinical article; controlled study; female; Helicobacter pylori; histopathology; human; human tissue; male; mucosa associated lymphoid tissue lymphoma; nonhuman; nucleotide sequence; priority journal; protein determination; protein isolation; sequence analysis; sequence homology; stomach carcinogenesis; stomach lymphoma; strain difference |
顯示於: | 微生物學科所 |
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