https://scholars.lib.ntu.edu.tw/handle/123456789/417219
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wang, Jin-Town | en_US |
dc.contributor.author | Chang C.-S. | en_US |
dc.contributor.author | Lee, Cha-Ze | en_US |
dc.contributor.author | JYH-CHIN YANG | en_US |
dc.contributor.author | Lin J.-T. | en_US |
dc.contributor.author | Wang T.-H. | en_US |
dc.creator | Wang T.-H.;Lin J.-T.;Yang J.-C.;Lee C.-Z.;Chang C.-S.;Wang, Jin-Town | - |
dc.date.accessioned | 2019-09-02T08:58:37Z | - |
dc.date.available | 2019-09-02T08:58:37Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032539757&doi=10.1006%2fbbrc.1998.8271&partnerID=40&md5=4fa0a838557652570ed5f7791cd687f2 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/417219 | - |
dc.description.abstract | This study attempted to identify a possible antibody response to Helicobacter pylori, which is associated with patients with adeno-carcinoma of the stomach. By using proteins of H. pylori as the antigen, pooled sera from gastric cancer and non-cancer patients were used as the first antibody for Western blot analysis. Antibody responses to a 26 kD secreted protein were observed in pooled cancer sera, but not in pooled sera from non-cancer patients. The protein was purified, while amino acid sequences revealed that it was a H. pylori species specific protein. The gene of this protein was cloned and a recombinant protein was expressed in E. coli. In addition, an antibody to the recombinant protein was tested in each individual patient using Western blot analysis. None of the forty non-gastric cancer patients were positive for the antibody to the recombinantly expressed 26 kD species specific protein. Meanwhile, six of the twenty four cancer patients tested positive (0/40 vs 6/24, p < 0.01). Results presented herein demonstrate that the species specific protein of H. pylori can be useful in detecting H. pylori associated with adenocarcinoma of the stomach. | - |
dc.language.iso | en_US | - |
dc.publisher | Academic Press Inc. | - |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | bacterium antibody; recombinant protein; adult; aged; amino acid sequence; antibody response; article; clinical article; controlled study; Escherichia coli; female; Helicobacter pylori; human; immunoblotting; male; molecular cloning; priority journal; protein purification; stomach adenocarcinoma; Taiwan; Bacteria (microorganisms); Escherichia coli; Helicobacter pylori | - |
dc.title | Antibody to a Helicobacter pylori species specific antigen in patients with adenocarcinoma of the stomach | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1006/bbrc.1998.8271 | - |
dc.identifier.pmid | 9514929 | - |
dc.identifier.scopus | 2-s2.0-0032539757 | - |
dc.relation.pages | 360-363 | - |
dc.relation.journalvolume | 244 | - |
dc.relation.journalissue | 2 | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-1595-6801 | - |
crisitem.author.orcid | 0000-0002-0974-3527 | - |
crisitem.author.orcid | 0000-0003-4219-0685 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 微生物學科所 |
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