https://scholars.lib.ntu.edu.tw/handle/123456789/424758
Title: | PAR-TERRA directs homologous sex chromosome pairing | Authors: | HSUEH-PING CHU Froberg, John E Kesner, Barry Oh, Hyun Jung Ji, Fei Sadreyev, Ruslan Pinter, Stefan F Lee, Jeannie T |
Issue Date: | Aug-2017 | Publisher: | NATURE PUBLISHING GROUP | Journal Volume: | 24 | Journal Issue: | 8 | Start page/Pages: | 620 | Source: | Nature structural & molecular biology | Abstract: | In mammals, homologous chromosomes rarely pair outside meiosis. One exception is the X chromosome, which transiently pairs during X-chromosome inactivation (XCI). How two chromosomes find each other in 3D space is not known. Here, we reveal a required interaction between the X-inactivation center (Xic) and the telomere in mouse embryonic stem (ES) cells. The subtelomeric, pseudoautosomal regions (PARs) of the two sex chromosomes (X and Y) also undergo pairing in both female and male cells. PARs transcribe a class of telomeric RNA, dubbed PAR-TERRA, which accounts for a vast majority of all TERRA transcripts. PAR-TERRA binds throughout the genome, including to the PAR and Xic. During X-chromosome pairing, PAR-TERRA anchors the Xic to the PAR, creating a 'tetrad' of pairwise homologous interactions (Xic-Xic, PAR-PAR, and Xic-PAR). Xic pairing occurs within the tetrad. Depleting PAR-TERRA abrogates pairing and blocks initiation of XCI, whereas autosomal PAR-TERRA induces ectopic pairing. We propose a 'constrained diffusion model' in which PAR-TERRA creates an interaction hub to guide Xic homology searching during XCI. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/424758 | ISSN: | 1545-9993 1545-9985 |
DOI: | https://api.elsevier.com/content/abstract/scopus_id/85026923350 10.1038/nsmb.3432 |
Appears in Collections: | 分子與細胞生物學研究所 |
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nsmb_chu.3432.pdf | 6.61 MB | Adobe PDF | View/Open |
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