https://scholars.lib.ntu.edu.tw/handle/123456789/425205
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Twu, Nae-Fang | en_US |
dc.contributor.author | Srinivasan, Ramanujam | en_US |
dc.contributor.author | CHUNG-HSI CHOU | en_US |
dc.contributor.author | LEANG-SHIN WU | en_US |
dc.contributor.author | CHIH-HSIEN CHIU | en_US |
dc.creator | CHIH-HSIEN CHIU;LEANG-SHIN WU;CHUNG-HSI CHOU;Srinivasan, Ramanujam;Twu, Nae-Fang | - |
dc.date.accessioned | 2019-09-26T07:08:57Z | - |
dc.date.available | 2019-09-26T07:08:57Z | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 09402993 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/425205 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-84857651323&partnerID=MN8TOARS | - |
dc.description.abstract | Cantharidin and its analog norcantharidin are active constituents of Mylabris, have been demonstrated to ailments for a variety of cancers. But several reports of cantharidin's natural or accidental toxicoses in field animals and humans showed a strong connection between cantharidin and its abortifacient and aphrodisiac properties. However, their exact cellular mechanisms in steroidogenesis remains poorly understood. Thus this study was aimed to explore the effects of cantharidin on luteal cell steroidogensis and to compare its effect with that of norcantharidin. For this purpose, luteal cells isolated from corpora lutea of native Taiwan goats were maintained in vitro and treated for 4 and 24 h with cantharidin and norcantharidin (0.1, 1.0, and 10 μg ml(-1)) to assess their steroidogenic effects. Progesterone (P(4)) levels and steroidogenic enzyme expression were assessed by enzyme immunoassay and Western blot methods, respectively. In caprine luteal cells, cantharidin and norcantharidin repressed basal P(4) production, as well as that mediated by ovine luteinizing hormone (oLH), 8-bromo-cyclic AMP (8-Br-cAMP), 22R-hydroxycholesterol (22R-OHC) and pregnenolone (P(5)). They also inhibited the expression of steroidogenic acute regulatory (StAR) protein, cytochrome P450 cholesterol side-chain cleavage (P450scc) enzyme, and 3β-hydroxysteroid dehydrogenase (3β-HSD) enzyme. Additionally, the greater inhibitory effect was detected using cantharidin, when it is compared with that of norcantharidin. Our results suggest that ingestion of cantharidin may decrease luteal steroidogenesis, and the decline in luteal P(4) levels may disrupt reproductive functions in humans as well as animals. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER GMBH, URBAN & FISCHER VERLAG | en_US |
dc.relation.ispartof | Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie | en_US |
dc.subject | Cantharidin; Norcantharidin; Caprine; Luteal cells; Steroidogenesis | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | 22 hydroxycholesterol; 3(or 17)beta hydroxysteroid dehydrogenase; 8 bromo cyclic AMP; cantharidin; cholesterol monooxygenase (side chain cleaving); luteinizing hormone; norcantharidin; pregnenolone; progesterone; steroidogenic acute regulatory protein; animal cell; article; cell proliferation; cell viability; controlled study; cytotoxicity test; down regulation; enzyme immunoassay; female; goat; in vitro study; luteal cell; nonhuman; protein expression; protein synthesis inhibition; steroidogenesis; toxicokinetics; toxin analysis; toxin synthesis; Western blotting; 3-Hydroxysteroid Dehydrogenases; Animals; Bicyclo Compounds, Heterocyclic; Blotting, Western; Cantharidin; Cell Culture Techniques; Cell Line; Cholesterol Side-Chain Cleavage Enzyme; Dose-Response Relationship, Drug; Female; Goats; Luteal Cells; Phosphoproteins; Progesterone; Animalia; Capra; Capra hircus; Mylabris; Ovis | - |
dc.title | Cantharidin and norcantharidin inhibit caprine luteal cell steroidogenesis in vitro | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.etp.2010.06.003 | - |
dc.identifier.pmid | 20594813 | - |
dc.identifier.scopus | 2-s2.0-84857651323 | - |
dc.identifier.isi | WOS:000299030400006 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84857651323 | - |
dc.relation.pages | 37 | en_US |
dc.relation.journalvolume | 64 | en_US |
dc.relation.journalissue | 1-2 | en_US |
dc.relation.pageend | 44 | en_US |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.dept | Office of Student Affairs | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.orcid | 0000-0002-4983-2786 | - |
crisitem.author.orcid | 0000-0002-9384-2527 | - |
crisitem.author.orcid | 0000-0002-1209-3852 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Administrative Unit | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 動物科學技術學系 |
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