https://scholars.lib.ntu.edu.tw/handle/123456789/425343
Title: | Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase antibodies for universal detection | Authors: | CHIH-HSIEN CHIU HEN-WEI WEI LEANG-SHIN WU |
Keywords: | steroidogenesis; P450scc; 3 beta-HSD; polyclonal antibody; bovine corpus luteum | Issue Date: | 2008 | Publisher: | TAYLOR & FRANCIS INC | Journal Volume: | 29 | Journal Issue: | 2 | Start page/Pages: | 152 | Source: | Journal of immunoassay & immunochemistry | Abstract: | The biosynthesis of steroids from steroidogenic cells are catalyzed by the two major enzymes, P450 side-chain cleavage enzyme (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD). This article describes the construction of two novel polyclonal antibodies against conserved recombinant protein and the validation of these antibodies on fixed tissue sections of bovine corpus luteum. The polyclonal antibodies were used successfully in Western blots and specifically reacted with P450scc and 3beta-HSD protein in bovine luteal cell extracts. Thus, P450scc and 3beta-HSD are two specific polyclonal antibodies that are integral products in the investigation of the biological function and regulatory mechanism involved in steroidogenesis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/425343 | ISSN: | 1532-1819 1532-4230 |
DOI: | 10.1080/15321810801887839 |
Appears in Collections: | 動物科學技術學系 |
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