https://scholars.lib.ntu.edu.tw/handle/123456789/425343
DC Field | Value | Language |
---|---|---|
dc.contributor.author | CHIH-HSIEN CHIU | en_US |
dc.contributor.author | HEN-WEI WEI | en_US |
dc.contributor.author | LEANG-SHIN WU | en_US |
dc.creator | LEANG-SHIN WU;HEN-WEI WEI;CHIH-HSIEN CHIU | - |
dc.date.accessioned | 2019-09-26T07:48:53Z | - |
dc.date.available | 2019-09-26T07:48:53Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1532-1819 | - |
dc.identifier.issn | 1532-4230 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/425343 | - |
dc.description.abstract | The biosynthesis of steroids from steroidogenic cells are catalyzed by the two major enzymes, P450 side-chain cleavage enzyme (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD). This article describes the construction of two novel polyclonal antibodies against conserved recombinant protein and the validation of these antibodies on fixed tissue sections of bovine corpus luteum. The polyclonal antibodies were used successfully in Western blots and specifically reacted with P450scc and 3beta-HSD protein in bovine luteal cell extracts. Thus, P450scc and 3beta-HSD are two specific polyclonal antibodies that are integral products in the investigation of the biological function and regulatory mechanism involved in steroidogenesis. | en_US |
dc.language.iso | en | en_US |
dc.publisher | TAYLOR & FRANCIS INC | en_US |
dc.relation.ispartof | Journal of immunoassay & immunochemistry | en_US |
dc.subject | steroidogenesis; P450scc; 3 beta-HSD; polyclonal antibody; bovine corpus luteum | en_US |
dc.title | Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase antibodies for universal detection | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1080/15321810801887839 | - |
dc.identifier.pmid | 18360810 | - |
dc.identifier.scopus | 2-s2.0-41149131737 | - |
dc.identifier.isi | WOS:000254298000004 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/41149131737 | - |
dc.relation.pages | 152 | en_US |
dc.relation.journalvolume | 29 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pageend | 160 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.orcid | 0000-0002-1209-3852 | - |
crisitem.author.orcid | 0000-0001-9096-1013 | - |
crisitem.author.orcid | 0000-0002-9384-2527 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
Appears in Collections: | 動物科學技術學系 |
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