|Title:||Fluctuating reaction rate and non-exponential blinking statistics in single-enzyme kinetics||Authors:||Tang J.
|Issue Date:||2008||Journal Volume:||463||Journal Issue:||1-3||Start page/Pages:||134-138||Source:||Chemical Physics Letters||Abstract:||
Extending the Michaelis-Menten kinetic scheme, we consider a three-state diffusion-controlled reaction model to investigate the effects of fluctuating reaction rate on the blinking statistics of single-enzyme catalytic reactions. As a result of conformational changes, the barrier-height and the reaction rate for the bottleneck enzymatic reaction could fluctuate in time, leading to non-exponential blinking statistics. To illustrate model applications, some reported experimental data for single £]-galactosidase molecules were reanalyzed here to extract useful kinetic parameters. ? 2008 Elsevier B.V.
|Appears in Collections:||高分子科學與工程學研究所|
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