https://scholars.lib.ntu.edu.tw/handle/123456789/425793
Title: | Effects of substrate-coating materials on the wound-healing process | Authors: | KAI-YIN LO Sun, Yung Shin Lin, Jin Young |
Keywords: | Cell migration | Fibroblasts | Surface coating | Wound-healing assay | Wound-healing rate | Issue Date: | 1-Jan-2019 | Journal Volume: | 12 | Journal Issue: | 7 | Source: | Materials | Abstract: | © 2019 by the authors. The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it difficult to compare results from different assays. In this paper, a modified barrier wound-healing assay was reported for studying the wound-healing process on different substrates in one single petri dish. In short, half of a dish was covered with the tape, and coating materials, poly-l-lysine and gelatin, were applied to the surface. After peeling off the tape, half of the surface was coated with the desired material. Then a customized barrier was placed inside the dish to create the wound. The results indicated that surface coating did not affect cell proliferation/viability, and the wound-healing rate increased in coated surfaces compared to uncoated ones. The present study provides a platform for further understanding the mechanisms of substrate coating-dependent wound-healing processes. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/425793 | DOI: | 10.3390/ma12172775 |
Appears in Collections: | 農業化學系 |
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