https://scholars.lib.ntu.edu.tw/handle/123456789/428812
標題: | A nanobiosensing method based on force measurement of antibody-antigen interaction for direct detection of enterovirus 71 by the chemically modified atomic force microscopic probe | 作者: | Hong, Chung Hung Hsieh, Chung Fan CHI-SHIN TSENG Huang, Wei Chih Guo, Cheng Yan SHI-MING LIN SI-CHEN LEE |
公開日期: | 1-十月-2017 | 出版社: | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | 卷: | 111 | 起(迄)頁: | 292 | 來源出版物: | Microbial Pathogenesis | 摘要: | © 2017 Elsevier Ltd Hand, Foot and mouth disease (HFMD) is a common disease with high infectivity for children, and enterovirus 71 (EV71) is one of the main pathogens to cause the type of illness. Therefore, the aim of this study was to propose a rapid and effective technique for detecting EV71 directly based on the mechanism of biological intermolecular force by using atomic force microscopy (AFM). At first, we coated EV71 particles on the mica surface and made the EV71 antibodies (anti-EV71) fixed on the AFM tip by means of several chemical procedures. Then, AFM chemically modified tip was applied to measure the unbinding forces between EV71 and anti-EV71 by contact mode. Finally, by using AFM imaging calculating software, the EV71 particle size (mean±SD) was 31.36±3.87 nm (n = 200) and this result was concordance with previous literature. Besides, the force (mean±SD) between EV71 antigen and antibody complex was 336.9±64.7 pN. The force (mean±SD) between anti-EV71 and non-specific specimens was 47.1±15.1 pN and was significantly smaller (P < 0.05). Apparently, the results show that we can precisely identify EV71 infection among the samples by measuring the force magnitude and observing the occurrence of EV71/anti-EV71 unbinding events. Therefore, the combination of AFM system and the chemically modified tip has the potential to be a rapid and effective method for detecting EV71 directly. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/428812 | ISSN: | 08824010 | DOI: | 10.1016/j.micpath.2017.08.051 | SDG/關鍵字: | antibody antigen interaction; antigen detection; Article; atomic force microscopy; binding affinity; biosensor; Enterovirus A71; medical parameters; nonhuman; priority journal; protein binding; protein cleavage; reverse transcription polymerase chain reaction; surface area; virus culture; virus detection; virus particle; atomic force microscopy; chemistry; devices; Enterovirus A; Enterovirus infection; evaluation study; genetic procedures; human; immunology; isolation and purification; procedures; virology; virus antibody; virus antigen; Antibodies, Viral; Antigens, Viral; Biosensing Techniques; Enterovirus A, Human; Enterovirus Infections; Humans; Microscopy, Atomic Force |
顯示於: | 醫療器材與醫學影像研究所 |
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