https://scholars.lib.ntu.edu.tw/handle/123456789/429245
Title: | Applying gene set analysis to characterize the activities of immune cells in estrogen receptor positive breast cancer. | Authors: | Yi-Hsuan Chang Yu-Chiao Chiu1 Yu-Ching Hsu Hui-Mei Tsai Tzu-Hung Hsiao ERIC YAO-YU CHUANG |
Keywords: | Breast cancer; Estrogen receptor (ER); Gene expression; Gene set; Survival analysis; Tumor infiltrating lymphocyte (TIL) | Issue Date: | 2016 | Source: | Translational Cancer Research | Abstract: | Background: Estrogen receptor (ER) is a crucial biomarker for subtyping breast cancer. The present study aimed to understand the influence of infiltrated immune cells to patients' outcome in estrogen receptor positive (ER+) breast cancer. Methods: Gene expression profiles of three breast cancer cohorts downloaded from Gene Expression Omnibus (GEO) were used in this study. We utilized gene set enrichment analysis (GSEA) to estimate the activities of immune cell infiltration based on 31 published immune gene sets. Each gene set was tested for ER+ associated prognostic value. GSEA was applied to identify biological functions associated with prognostic immune gene sets. Results: Nine subtypes of immune cells showed ER+ specific association with patient survival; seven of them formed two co-activation clusters, including: (I) activated CD4, CD8, effector memory CD4, and (II) regulatory T cell, dendritic cell, eosinophil, and mast cell, substantially representing innate and adaptive immunity. Among them, activated CD8 and mast cell were independent prognostic factors in multivariate Cox regression. Functional annotation analysis revealed their involvement in breast cancer subtyping, relapse, and metastasis. Conclusions: We devised a gene set analysis to comprehensively investigate the involvement of ER specific immune cell activities and prognosis in breast cancer. Our work provides hints of the interaction between infiltrated immune cells and activated oncogene in ER+ breast cancer and may contribute to the biological basis for the development of immunotherapy. ? Translational Cancer Research. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966430554&doi=10.21037%2ftcr.2016.04.09&partnerID=40&md5=9d7b5ee352e16d9d9f8d5462df03bf97 | ISSN: | 2218676x | DOI: | 10.21037/tcr.2016.04.09 |
Appears in Collections: | 生醫電子與資訊學研究所 |
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