https://scholars.lib.ntu.edu.tw/handle/123456789/429719
標題: | Exocytosis of gold nanoparticle and photosensitizer from cancer cells and their effects on photodynamic and photothermal processes | 作者: | Yulu He Wei-Hsiang Hua Meng-Chun Low Yu-Hsuan Tsai Cheng-Jin Cai Hsin-Chun Chiang Jian-He Yu Jen-Hung Hsiao Po-Hao Tseng Yean-Woei Kiang C. C. Yang Zhenxi Zhang YEAN-WOEI KIANG |
公開日期: | 2018 | 卷: | 29 | 期: | 23 | 起(迄)頁: | 24460-24467 | 來源出版物: | Nanotechnology | 摘要: | We first illustrate the faster decrease of the photothermal (PT) effect with the delay time of laser treatment, in which the illumination of a 1064 nm laser effectively excites the localized surface plasmon (LSP) resonance of cell-up-taken gold nanoring (NRI) linked with a photosensitizer (PS), when compared with the photodynamic (PD) effect produced by the illumination of a 660 nm laser for effective PS excitation. The measurement results of the metal contents of Au NRI and PS based on inductively coupled plasma mass spectroscopy and the PS fluorescence intensity based on flow cytometry show that the linkage of NRI and PS is rapidly broken for releasing PS through the effect of glutathione in lysosome after cell uptake. Meanwhile, NRI escapes from a cell with a high rate such that the PT effect decays fast while the released PS can stay inside a cell longer for producing a prolonged PD effect. The effective delivery of PS through the linkage with Au NRI for cell uptake and the advantageous effect of LSP resonance at a PS absorption wavelength on the PD process are also demonstrated. ? 2018 IOP Publishing Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/429719 | ISSN: | 9574484 | DOI: | 10.1088/1361-6528/aab933 | SDG/關鍵字: | Cells; Gold nanoparticles; Inductively coupled plasma; Laser excitation; Mass spectrometry; Metal nanoparticles; Molecular biology; Photodynamic therapy; Photosensitizers; Surface plasmons; Absorption wavelengths; endocytosis; exocytosis; Fluorescence intensities; Inductively coupled plasma mass spectroscopies; Localized surface plasmon; Photothermal process; Photothermal therapy; Cytology; gold; metal nanoparticle; photosensitizing agent; atomic absorption spectrometry; chemistry; drug effect; endocytosis; exocytosis; fluorescence; human; laser; photochemotherapy; surface plasmon resonance; thermotherapy; tumor cell line; Cell Line, Tumor; Endocytosis; Exocytosis; Fluorescence; Gold; Humans; Hyperthermia, Induced; Lasers; Metal Nanoparticles; Photochemotherapy; Photosensitizing Agents; Spectrophotometry, Atomic; Surface Plasmon Resonance |
顯示於: | 光電工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。