https://scholars.lib.ntu.edu.tw/handle/123456789/431102
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHENG-CHUNG FANG | en_US |
dc.contributor.author | TZUNG-HSIN CHOU | en_US |
dc.contributor.author | JENQ-WEN HUANG | en_US |
dc.contributor.author | CHIEN-CHANG LEE | en_US |
dc.contributor.author | SHYR-CHYR CHEN | en_US |
dc.creator | JENQ-WEN HUANG;SHYR-CHYR CHEN;CHIEN-CHANG LEE;Chou, Tzung Hsin;CHENG-CHUNG FANG | - |
dc.date.accessioned | 2019-11-12T02:22:57Z | - |
dc.date.available | 2019-11-12T02:22:57Z | - |
dc.date.issued | 2018-06-21 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/431102 | - |
dc.description.abstract | Peritoneal adhesions develop after abdominal surgery, trauma or intraperitoneal infections, and have important consequences. The deposition of peritoneal fibrin is a common pathophysiological pathway for the formation of adhesions. Here, we aimed to examine the effects of fibrin-induced cytokine production on peritoneal mesothelial cells (PMCs), and to block the effects of fibrin using an integrin-linked kinase (ILK) inhibitor, QLT-0267. PMCs were cultured from the enzymatic disaggregation of rat omentum. After the PMCs were covered with fibrin, the expression of IL-1β, IL-6, TNFα and VEGF-A increased. This increase in cytokine production was attenuated by QLT-0267, which acted via the inhibition of both the ILK and focal adhesion kinase (FAK) pathways, and subsequently via the GSK-3β pathway. We found that QLT-0267 decreased both the severity of peritoneal adhesion and the serum levels of IL-6 in our post-surgical adhesion mouse model. In conclusion, our study provides novel evidence that fibrin-induced cytokine production may involve in the mechanism of peritoneal adhesion formation. Furthermore, the use of the small molecule inhibitor QLT-0267 is a new strategy in preventing peritoneal adhesion in patients undergoing abdominal surgery. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.relation.ispartof | Scientific reports | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antiinflammatory agent; azo compound; cytokine; fibrin; integrin-linked kinase; protein kinase inhibitor; protein serine threonine kinase; pyrazole derivative; QLT 0267; animal; cell culture; drug effect; epithelium cell; genetics; Institute for Cancer Research mouse; male; metabolism; mouse; pathology; peritoneum; rat; tissue adhesion; Animals; Anti-Inflammatory Agents; Azo Compounds; Cells, Cultured; Cytokines; Epithelial Cells; Fibrin; Male; Mice; Mice, Inbred ICR; Peritoneum; Protein Kinase Inhibitors; Protein-Serine-Threonine Kinases; Pyrazoles; Rats; Tissue Adhesions | - |
dc.title | The Small Molecule Inhibitor QLT-0267 Decreases the Production of Fibrin-Induced Inflammatory Cytokines and Prevents Post-Surgical Peritoneal Adhesions | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/s41598-018-25994-5 | - |
dc.identifier.pmid | 29930281 | - |
dc.identifier.scopus | 2-s2.0-85072528225 | - |
dc.identifier.isi | WOS:000435790500060 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85072528225 | - |
dc.relation.journalvolume | 8 | en_US |
dc.relation.journalissue | 1 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Emergency Medicine | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.dept | Emergency Medicine | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Emergency Medicine | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.dept | Emergency Medicine | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.orcid | 0000-0001-5386-6674 | - |
crisitem.author.orcid | 0000-0002-8145-3751 | - |
crisitem.author.orcid | 0000-0001-8011-2317 | - |
crisitem.author.orcid | 0000-0002-1243-2463 | - |
crisitem.author.orcid | 0000-0002-5858-3013 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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