https://scholars.lib.ntu.edu.tw/handle/123456789/431165
標題: | Cobalt chloride treatment induces autophagic apoptosis in human glioma cells via a p53-dependent pathway | 作者: | Cheng B.-C. Chen J.-T. Yang S.-T. Chio C.-C. SHING-HWA LIU Chen R.-M. |
關鍵字: | Autophagic Apoptosis; Autophagy; Hypoxia; Malignant Glioma; P53 | 公開日期: | 2017 | 出版社: | Spandidos Publications | 卷: | 50 | 期: | 3 | 起(迄)頁: | 964-974 | 來源出版物: | International Journal of Oncology | 摘要: | Malignant glioma is the most aggressive brain tumor. Hypoxic condition has been explored for killing cancer stem cells or drug-resistant tumor cells. This study investigated the effects of hypoxia on autophagic death and the possible mechanisms. Exposure of human malignant glioma U87-MG cells to cobalt chloride (CoCl2) increased cellular hypoxia-inducible factor-1α levels and concurrently decreased cell viability concentration-and time-dependently. In parallel, treatment with CoCl2 suppressed proliferation of human U87-MG cells. Autophagic cells and levels of LC3-II were concentration-and time-dependently induced in human U87-MG cells after exposure to CoCl2. However, pretreatment with 3-mehyladenine (3-MA) and chloroquine, inhibitors of cell autophagy, caused significant alleviations in CoCl2-induced cell autophagy. In contrast, exposure to rapamycin, an inducer of cell autophagy, synergistically induced hypoxiainduced autophagy of U87-MG cells. Administration of human U87-MG cells with CoCl2 triggered caspase-3 activation and cell apoptosis. Interestingly, pretreatment with 3-MA and chloroquine remarkably suppressed CoCl2-induced caspase-3 activation and cell apoptosis. Application of p53 small interference (si)RNA into human U87-MG cells downregulated levels of this protein and simultaneously lowered hypoxiaand 3-MA-induced alterations in cell autophagy, apoptosis, and death. The hypoxia-induced autophagy and apoptosis of DBTRG-05MG cells were significantly lowered by 3-MA pretreatment and p53 knockdown. Therefore, the present study shows that CoCl2 treatment can induce autophagy of human glioma cells and subsequent autophagic apoptosis via a p53-dependent pathway. Hypoxia-induced autophagic apoptosis may be applied as a therapeutic strategy for treatment of glioma patients. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013059734&doi=10.3892%2fijo.2017.3861&partnerID=40&md5=f183188645cd6d6fc84e9cb64bb9251f https://scholars.lib.ntu.edu.tw/handle/123456789/431165 |
ISSN: | 1019-6439 | DOI: | 10.3892/ijo.2017.3861 | SDG/關鍵字: | caspase 3; cobalt chloride; hypoxia inducible factor 1alpha; protein p53; 3-methyladenine; adenine; antimutagenic agent; caspase 3; chloroquine; cobalt; cobalt chloride; HIF1A protein, human; hypoxia inducible factor 1alpha; MAP1LC3A protein, human; microtubule associated protein; protein p53; rapamycin; reactive oxygen metabolite; small interfering RNA; TP53 protein, human; antineoplastic activity; antiproliferative activity; apoptosis; Article; autophagy; cell hypoxia; cell survival; cell viability; concentration response; controlled study; down regulation; drug cytotoxicity; drug mechanism; enzyme activation; glioma cell; human; human cell; analogs and derivatives; apoptosis; autophagy; Brain Neoplasms; cell proliferation; drug effects; genetics; glioma; metabolism; pathology; RNA interference; tumor cell line; Adenine; Antimutagenic Agents; Apoptosis; Autophagy; Brain Neoplasms; Caspase 3; Cell Hypoxia; Cell Line, Tumor; Cell Proliferation; Cell Survival; Chloroquine; Cobalt; Glioma; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Microtubule-Associated Proteins; Reactive Oxygen Species; RNA Interference; RNA, Small Interfering; Sirolimus; Tumor Suppressor Protein p53 |
顯示於: | 毒理學研究所 |
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