https://scholars.lib.ntu.edu.tw/handle/123456789/431400
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lee, Sze Hwei | en_US |
dc.contributor.author | SHEY-YING CHEN | en_US |
dc.contributor.author | JUNG-YIEN CHIEN | en_US |
dc.contributor.author | Lee, Tai Fen | en_US |
dc.contributor.author | JONG-MIN CHEN | en_US |
dc.contributor.author | PO-REN HSUEH | en_US |
dc.creator | PO-REN HSUEH;Chen, Jong Min;Lee, Tai Fen;Chien, Jung Yien;SHEY-YING CHEN;Lee, Sze Hwei | - |
dc.date.accessioned | 2019-11-18T06:01:44Z | - |
dc.date.available | 2019-11-18T06:01:44Z | - |
dc.date.issued | 2019-10-01 | - |
dc.identifier.issn | 16841182 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/431400 | - |
dc.description.abstract | © 2019 Background/purpose: Early recognition of causative pathogens is critical for the appropriate management of central nervous system infection and improved outcomes. The BioFire® FilmArray® Meningitis/Encephalitis Panel (BioFire® ME Panel, BioFire Diagnostics) is the first U.S. Food and Drug Administration (FDA)-approved multiplex PCR assay that allows the rapid detection of 14 pathogens, including bacteria (n = 6), viruses (n = 7), and fungi (n = 1), from cerebrospinal fluid (CSF). The performance of the panel is expected to be dependent on the epidemiology of M/E in different geographical regions. Methods: In this preliminary study, we used the BioFire® ME Panel in 42 subjects who presented to the emergency department with symptoms of M/E in our hospital. The results were compared to conventional culture, antigen detection, PCR, and various laboratory findings. Results: The panel detected six positive samples, of which five were viral and one bacterial. We observed an overall agreement rate of 88% between the BioFire® ME Panel results and the conventional methods. There were no false-positive findings, but five discordant results were observed for enterovirus, herpes simplex virus type 1, Escherichia coli, and Cryptococcus species. Conclusions: The BioFire® ME Panel performed equivalently to the traditional PCR methods for virus detection, and better than bacterial cultures. This revolutionary system represents a paradigm shift in the diagnosis of M/E and may aid in the rapid identification of community-acquired M/E. However, the usefulness of this tool is limited in regions with a high prevalence of infectious M/E caused by microorganisms not included in the panel. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER TAIWAN | en_US |
dc.relation.ispartof | Journal of Microbiology, Immunology and Infection | en_US |
dc.subject | BioFire ME panel ® | Emergency department | Encephalitis | Meningitis | Multiplex PCR assay | en_US |
dc.title | Usefulness of the FilmArray meningitis/encephalitis (M/E) panel for the diagnosis of infectious meningitis and encephalitis in Taiwan | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.jmii.2019.04.005 | - |
dc.identifier.pmid | 31085115 | - |
dc.identifier.scopus | 2-s2.0-85065451655 | - |
dc.identifier.isi | WOS:000487963100011 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85065451655 | - |
dc.relation.pages | 760 | en_US |
dc.relation.journalvolume | 52 | en_US |
dc.relation.journalissue | 5 | en_US |
dc.relation.pageend | 768 | en_US |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | Emergency Medicine | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-5855-6170 | - |
crisitem.author.orcid | 0000-0002-7718-0594 | - |
crisitem.author.orcid | 0000-0002-7502-9225 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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