|Title:||Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS||Authors:||SHEY-YING CHEN
Teng, Shih Hua
Wang, Xiao Mei
Lee, Tai Fen
Huang, Yu Chung
Liao, Chun Hsing
|Keywords:||Bruker Biotyper MALDI-TOF MS | classification model | matrix-assisted laser desorption/ionization-time of flight mass spectrometry | Staphylococcus argenteus | Staphylococcus aureus||Issue Date:||1-Jul-2018||Publisher:||FUTURE MEDICINE LTD||Journal Volume:||13||Journal Issue:||9||Start page/Pages:||997||Source:||Future Microbiology||Abstract:||
© 2018 Future Medicine Ltd. Aim: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. Materials & methods: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. Results: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. Conclusion: The classification model could accurately differentiate S. argenteus from MSSA.
|Appears in Collections:||醫學院附設醫院 (臺大醫院)|
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