https://scholars.lib.ntu.edu.tw/handle/123456789/431809
標題: | Two distinct arsenite-resistant variants of Leishmania amazonensis take different routes to achieve resistance as revealed by comparative transcriptomics | 作者: | Lin, YC Hsu, JY Jacob Shujui Hsu Chi, Y Chiang, SC Lee, ST |
關鍵字: | Arsenite resistance; Arsenate resistance; Leishmania; cDNA subtractive hybridization; DNA microarray | 公開日期: | 2008 | 出版社: | ELSEVIER SCIENCE BV | 卷: | 162 | 期: | 1 | 起(迄)頁: | 16 | 來源出版物: | Molecular And Biochemical Parasitology | 摘要: | Genome-wide search for the genes involved in arsenite resistance in two distinct variants A and A' of Leishmania amazonensis revealed that the two variants used two different mechanisms to achieve resistance, even though these two variants were derived from the same clone and selected against arsenite under the same conditions. In variant A, the variant with DNA amplification, the biochemical pathways for detoxification of oxidative stress, the energy generation system to support the biochemical and physiological needs of the variant for DNA and protein synthesis and the arsenite translocating system to dispose arsenite are among the primary biochemical events that are upregulated under the arsenite stress to gain resistance. In variant A', the variant without DNA amplification, the upregulation of aquaglyceroporin (AQP) gene and the high level of resistance to arsenate point to the direction that the resistance gained by the variant is due to arsenate which is probably oxidized from arsenite in the arsenite solution used for selection and the maintenance of the cell culture. As a result of the AQP upregulation for arsenite disposal, a different set of biochemical pathways for detoxification of oxidative stress, energy generation and cellular signaling are upregulated to sustain the growth of the variant to gain resistance to arsenate. From current evidences, reactive oxygen species (ROS) overproduced by the parasite soon after exposure to arsenite appear to play an instrumental role in both variants to initiate the subsequent biochemical events that allow the same clone of L. amazonensis to take two totally different routes to diverge into two different variants. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/431809 | ISSN: | 0166-6851 | DOI: | 10.1016/j.molbiopara.2008.06.015 |
顯示於: | 基因體暨蛋白體醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。