https://scholars.lib.ntu.edu.tw/handle/123456789/434762
標題: | Sodium thiosulfate enhances production of polysaccharides and anticancer activities of sulfated polysaccharides in Antrodia cinnamomea | 作者: | CHIA-CHUAN CHANG Lin, T.-Y.; Tseng, A.-J.; Chao, C.-H.; Lu, M.-K. |
關鍵字: | Anticancer activity; Antrodia cinnamomea; Sodium thiosulfate; Sulfated polysaccharides | 公開日期: | 2019 | 卷: | 216 | 起(迄)頁: | 204-212 | 來源出版物: | Carbohydrate Polymers | 摘要: | Sulfated polysaccharides (SPSs) are polysaccharides (PSs) with high sulfate functionalization and possess bioactivities. This study aimed to increase the sulfate content of SPSs in Antrodia cinnamomea through sulfate feeding. Feeding A. cinnamomea with sodium thiosulfate was found to increase yields of PSs and SPSs in A. cinnamomea. The SPSs thus obtained (ST-SPS) were further isolated, showing enhanced sulfate content of 2.5 mmol/g. Sodium thiosulfate induced changes in molecular weight from 320 kDa to 1342 kDa, and area percentage of low-molecular-weight ST-SPS (< 20 kDa) was decreased. Functional studies revealed that sodium thiosulfate increased the ST-SPS anticancer efficacy in cancer cells via inhibition of EGFR/AKT signaling. Moreover, the ST-SPS enhanced synergistically cisplatin-, gefitinib- and 5 FU-induced cytotoxic effects in lung cancer H1975 cells and colon cancer CT26 cells. This study is the first to demonstrate that sodium thiosulfate induced changes in properties of A. cinnamomea with the anticancer mechanisms of ST-SPS. ? 2019 Elsevier Ltd |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/434762 | DOI: | 10.1016/j.carbpol.2019.04.017 | SDG/關鍵字: | Cell signaling; Diseases; Molecular weight; Polysaccharides; Sulfur compounds; Anti-cancer mechanism; Anticancer activities; Antrodia cinnamomea; Cytotoxic effects; Functionalizations; Low molecular weight; Sodium thiosulfate; Sulfated polysaccharides; Sodium compounds; antineoplastic agent; cisplatin; epidermal growth factor receptor; fluorouracil; gefitinib; polysaccharide; sodium thiosulfate; sulfate; thiosulfate; animal; Antrodia; apoptosis; biosynthesis; chemistry; drug effect; drug potentiation; human; IC50; isolation and purification; metabolism; molecular weight; mouse; phosphorylation; signal transduction; tumor cell line; Animals; Antineoplastic Agents; Antrodia; Apoptosis; Cell Line, Tumor; Cisplatin; Drug Synergism; ErbB Receptors; Fluorouracil; Gefitinib; Humans; Inhibitory Concentration 50; Mice; Molecular Weight; Phosphorylation; Polysaccharides; Signal Transduction; Sulfuric Acid Esters; Thiosulfates |
顯示於: | 藥學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。