https://scholars.lib.ntu.edu.tw/handle/123456789/436296
標題: | Low-fluence rate, long duration photodynamic therapy in glioma mouse model using organic light emitting diode (OLED) | 作者: | Guo, H.-W. Lin, L.-T. Chen, P.-H. Ho, M.-H. Huang, W.-T. Lee, Y.-J. Chiou, S.-H. Hsieh, Y.-S. CHEN-YUAN DONG Wang, H.-W. |
關鍵字: | 5-Aminolevulinic acid (ALA); Gliomas; Metronomic photodynamic therapy (mPDT); Organic light emitting diode (OLED) | 公開日期: | 2015 | 卷: | 12 | 期: | 3 | 起(迄)頁: | 504-510 | 來源出版物: | Photodiagnosis and Photodynamic Therapy | 摘要: | Background: The treatment of gliomas poses significant clinical challenges due to resistance to chemo and radiation therapy, and treatment side effects. Metronomic photodynamic therapy (mPDT), which involves long treatment time with low fluence rate and multiple or continuous photosensitizer administrations, has potential in treating gliomas without threatening the quality of life and has been demonstrated in rats and rabbits. mPDT in small animals such as mouse is not yet shown due to lack of lightweight illumination device for long periods of time. Methods: We presented low fluence rate (3mW/cm2) and long duration (3.7h) PDT treatment in a nude mouse model of human glioblastoma by using organic light emitting diode (OLED) with single dose of 5-aminolevulinic acid (ALA) administration as photosensitizer. Tumor volume was measured using bioluminescent imaging and the animal survival time was recorded. Additionally, we have performed limited PDT dosimetric measurements of PpIX fluorescence, tumor oxygenation and hemoglobin concentration in 3 PDT mice. Results: For animals with similar pre- and immediate post-light tumor volume, the averaged total survival time of PDT mice is 40.5 ± 9.2 days that are significantly longer than the control mice (26.0 ± 2.0 days). The post-light survival time of PDT mice is 14.3 ± 5.9 days that are marginally longer than the control group (8.0 ± 0.0 days). In the dosimetric measurement, good maintenance of PpIX fluorescence in one PDT mouse has relatively improved survival time, compared with the other two PDT mice (i.e., 24 days versus 16 and 17 days). Conclusions: This pilot study demonstrated the feasibility of low-fluence rate and long treatment time of ALA-PDT using OLED without anesthetization of animals. The response of PDT treated animals with similar pre- and post-light tumor volume is encouraging to show a longer survival time than the controls. The dosimetric indices such as photosensitizer fluorescence and tissue oxygenation would help understand the possible treatment barriers for further improvement of treatment plans. ? 2015 Elsevier B.V. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/436296 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940895330&doi=10.1016%2fj.pdpdt.2015.04.007&partnerID=40&md5=44d59b72238a24cdf8cb902c636c9e1d |
ISSN: | 15721000 | DOI: | 10.1016/j.pdpdt.2015.04.007 | SDG/關鍵字: | aminolevulinic acid; aminolevulinic acid; photosensitizing agent; protoporphyrin; animal experiment; animal model; animal tissue; Article; controlled study; dosimetry; fluorescence; glioblastoma; human; human cell; light emitting diode; metronomic photodynamic therapy; mouse; nonhuman; organic light emitting diode; photodynamic therapy; priority journal; single drug dose; survival time; tissue oxygenation; treatment duration; tumor volume; tumor xenograft; animal; disease model; glioblastoma; nude mouse; photochemotherapy; pilot study; procedures; survival analysis; time factor; Aminolevulinic Acid; Animals; Disease Models, Animal; Glioblastoma; Humans; Mice; Mice, Nude; Photochemotherapy; Photosensitizing Agents; Pilot Projects; Protoporphyrins; Survival Analysis; Time Factors |
顯示於: | 物理學系 |
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