https://scholars.lib.ntu.edu.tw/handle/123456789/436318
標題: | Quantitative analysis of multiphoton excitation autofluorescence and second harmonic generation imaging for medical diagnosis | 作者: | Chen, W.-L. Hu, P.-S. Ghazaryan, A. Chen, S.-J. Tsai, T.-H. CHEN-YUAN DONG |
關鍵字: | Image analysis; Nonlinear optics; Optical microscopy; Second harmonic generation; Two-photon | 公開日期: | 2012 | 卷: | 36 | 期: | 7 | 起(迄)頁: | 519-526 | 來源出版物: | Computerized Medical Imaging and Graphics | 摘要: | In recent years, two-photon excitation fluorescence and second harmonic generation microscopy has become an important tool in biomedical research. The ability of two-photon microscopy to achieve optical sectioning with minimal invasiveness is particularly advantageous for biomedical diagnosis. Advances in the miniaturization of the imaging system have increased its clinical potential, together with the development of quantitative technique for the analysis of data acquired using these imaging modalities. We present a review of the quantitative analysis techniques that have been used successfully with two-photon excitation fluorescence and SHG imaging. Specifically, quantification techniques using ratiometric, morphological, and structural differences to analyze two-photon images will be discussed, and their effectiveness at evaluating dermal and corneal pathologies and cancerous tumor growth will be described. ? 2012 Elsevier Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/436318 | DOI: | 10.1016/j.compmedimag.2012.06.003 | SDG/關鍵字: | Analysis of data; Autofluorescence; Biomedical research; Cancerous tumors; Corneal pathologies; Imaging modality; Invasiveness; Multiphoton excitation; Optical sectioning; Quantitative techniques; Ratiometric; Second harmonic generation microscopies (SHG); Second harmonics; Structural differences; Two photon; Two photon excitation fluorescence; Two photon microscopy; Biomedical engineering; Diagnosis; Fluorescence; Harmonic generation; Image analysis; Optical microscopy; Photons; Nonlinear optics; autofluorescence imaging; basal cell carcinoma; cancer growth; cornea disease; elastolysis; electronics; fluorescence microscopy; Fourier transformation; human; keloid; lung adenocarcinoma; lung squamous cell carcinoma; medical research; minimally invasive procedure; morphea; multiphoton microscopy; nonhuman; optical coherence tomography; priority journal; quantitative analysis; review; second harmonic generation; Equipment Design; Evaluation Studies as Topic; Image Processing, Computer-Assisted; Microscopy, Fluorescence, Multiphoton; Photons |
顯示於: | 物理學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。