https://scholars.lib.ntu.edu.tw/handle/123456789/436384
標題: | Combination of multiphoton and reflective confocal imaging of cornea | 作者: | Chen, W.-L. Sun, Y. Lo, W. Tan, H.-Y. CHEN-YUAN DONG |
關鍵字: | Confocal microscopy; Multiharmonic generation; Multiphoton processes; Ophthalmology; Three-dimensional microscopy | 公開日期: | 2008 | 卷: | 71 | 期: | 2 | 起(迄)頁: | 83-85 | 來源出版物: | Microscopy Research and Technique | 摘要: | We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic generation signal is used to detect collagen, found mostly in the stroma of the cornea. The reflective confocal imaging allows detection of epithelial cells and keratocytes in the stroma. The system is first tested on bovine cornea. Assessment of the result on the bovine eye will be used to evaluate the potential of the system as a technique for in vivo clinical application. © 2007 Wiley-Liss, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/436384 https://www.scopus.com/inward/record.uri?eid=2-s2.0-39449106610&doi=10.1002%2fjemt.20528&partnerID=40&md5=8d6e53f812af3d092eef80fb82dd2fef |
ISSN: | 1059910X | DOI: | 10.1002/jemt.20528 | SDG/關鍵字: | collagen; animal tissue; article; autofluorescence; cell ultrastructure; confocal microscopy; cornea cell; cornea stroma; cow; epithelium cell; imaging system; minimally invasive surgery; multiphoton microscopy; nonhuman; priority journal; Animals; Cattle; Cornea; Corneal Stroma; Epithelial Cells; Image Processing, Computer-Assisted; Keratinocytes; Microscopy, Confocal; Bovinae |
顯示於: | 物理學系 |
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