https://scholars.lib.ntu.edu.tw/handle/123456789/444433
標題: | Areca-nut extract modulates antigen-specific immunity and augments inflammation in ovalbumin-sensitized mice | 作者: | Wang, Chia-Chi Lin, Hung-Li Wey, Shiaw-Pyng TONG-RONG JAN CHIA-CHI WANG |
公開日期: | 2011 | 卷: | 33 | 期: | 2 | 起(迄)頁: | 315-322 | 來源出版物: | Immunopharmacology and Immunotoxicology | 摘要: | Areca-nut chewing has been linked to oral cancer and many other diseases, in which immune deterioration and tissue inflammation are plausibly involved. Recent studies reported that areca-nut extract (ANE) affected the functionality of lymphocytes and neutrophils in vitro. In the present study, we investigated the immunomodulatory effect of ANE in vivo. Ovalbumin (OVA)-sensitized mice were daily administered with ANE (5-50mg/kg) for 10 doses by intraperitoneal injection from days 1 to 5 and from 8 to 12. The mice were systemically sensitized with OVA on day 3, and their footpads were challenged with OVA to induce delayed-type hypersensitivity (DTH) reactions on day 13. The serum level of OVA-specific IgM and IgG1 was significantly attenuated by 5 and 25mg/kg of ANE, whereas OVA-specific IgG2a was markedly enhanced by 50mg/kg of ANE. The production of interferon (IFN)-γ by splenocytes reexposed to OVA in culture was markedly augmented by ANE (25 and 50mg/kg). In addition, ANE (25 and 50mg/kg) demonstrated an enhancing effect on DTH reactions, including the tissue swelling, the infiltration of CD3 + and F4/80 + cells, and the expression of IFN-γ and tumor necrosis factor (TNF)-α in the footpads challenged with OVA. The phagocytic activity and TNF-α production by the splenic CD11b + cells were also enhanced in ANE-treated groups. Taken together, these results demonstrated that ANE modulated antigen-specific immune responses and promoted inflammatory reactions in vivo, which may contribute to immune deregulation associated with areca-related diseases. ? 2011 Informa Healthcare USA, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/444433 | ISSN: | 1532-2513 | DOI: | 10.3109/08923973.2010.507208 | SDG/關鍵字: | Areca catechu extract; gamma interferon; immunoglobulin G1; immunoglobulin G2a; immunoglobulin M; immunomodulating agent; ovalbumin; peanut extract; tumor necrosis factor alpha; unclassified drug; animal experiment; animal model; antigen specificity; article; betel nut; CD3+ T lymphocyte; edema; foot pad; hypersensitivity; immunity; immunoglobulin blood level; immunomodulation; in vivo study; inflammation; interferon production; male; mouse; nonhuman; phagocytosis; priority journal; spleen cell; Adjuvants, Immunologic; Animals; Antigens, Plant; Areca; Epitopes; Inflammation Mediators; Male; Mice; Mice, Inbred BALB C; Nuts; Ovalbumin; Phagocytosis; Plant Extracts; Random Allocation; Up-Regulation; Areca; Areca catechu; Mus |
顯示於: | 獸醫學系 |
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