https://scholars.lib.ntu.edu.tw/handle/123456789/446064
標題: | Gene variations in cis-acting elements between the taiwan and prototype strains of porcine epidemic diarrhea virus alter viral gene expression | 作者: | Tsai T.-L. Su C.-C. Hsieh C.-C. Lin C.-N. HUI-WEN CHANG Lo C.-Y. Lin C.-H. Wu H.-Y. |
關鍵字: | Cis-acting element; Gene evolution; Gene expression; Nucleotide composition; Porcine epidemic diarrhea virus | 公開日期: | 2018 | 卷: | 9 | 期: | 12 | 來源出版物: | Genes | 摘要: | In 2013, the outbreak of porcine epidemic diarrhea (PED) in Taiwan caused serious economic losses. In this study, we examined whether the variations of the cis-acting elements between the porcine epidemic diarrhea virus (PEDV) Taiwan (TW) strain and the prototype strain CV777 alter gene expression. For this aim, we analyzed the variations of the cis-acting elements in the 5’ and 3’ untranslated regions (UTRs) between the PEDV TW, CV777, and other reference strains. We also determined the previously unidentified transcription regulatory sequence (TRS), a sequence motif required for coronavirus transcription, and found that a nucleotide deletion in the TW strain, in comparison with CV777 strain, immediately downstream of the leader core sequence alters the identity between the leader TRS and the body TRS. Functional analyses using coronavirus defective interfering (DI) RNA revealed that such variations in cis-acting elements for the TW strain compared with the CV777 strain have an influence on the efficiency of gene expression. The current data show for the first time the evolution of PEDV in terms of cis-acting elements and their effects on gene expression, and thus may contribute to our understanding of recent PED outbreaks worldwide. ? 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/446064 | ISSN: | 20734425 | DOI: | 10.3390/genes9120591 | SDG/關鍵字: | cis acting element; 3' untranslated region; 5' untranslated region; Article; comparative study; controlled study; defective virus; downstream processing; epidemic; gene deletion; gene expression; gene mutation; gene structure; genetic variation; nonhuman; Porcine epidemic diarrhea virus; regulatory sequence; RNA interference; sequence alignment; Taiwan; virus gene; virus strain |
顯示於: | 分子暨比較病理生物學研究所 |
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