https://scholars.lib.ntu.edu.tw/handle/123456789/446393
標題: | Activation of amygdala opioid receptors by electroacupuncture of Feng-Chi (GB20) acupoints exacerbates focal epilepsy | 作者: | Yi P.-L. Lu C.-Y. Cheng C.-H. Tsai Y.-F. Lin C.-T. FANG-CHIA CHANG CHUNG-TIEN LIN |
公開日期: | 2013 | 卷: | 13 | 來源出版物: | BMC Complementary and Alternative Medicine | 摘要: | Background: The effect of seizure suppression by acupuncture of Feng-Chi (GB20) acupoints has been documented in the ancient Chinese literature, Lingshu Jing (Classic of the Miraculous Pivot), however, there is a lack of scientific evidence to prove it. This current study was designed to elucidate the effect of electroacupuncture (EA) stimulation of bilateral Feng-Chi (GB20) acupoints on the epileptic activity by employing an animal model of focal epilepsy.Methods: Administration of pilocarpine into the left central nucleus of amygdala (CeA) induced the focal epilepsy in rats. Rats received a 30-min 100?Hz EA stimulation of bilateral Feng-Chi acupoints per day, beginning at 30?minutes before the dark period and performing in three consecutive days. The broad-spectrum opioid receptor antagonist (naloxone), μ-receptor antagonist (naloxonazine), δ-receptor antagonist (naltrindole) and κ-receptor antagonist (nor-binaltorphimine) were administered directly into the CeA to elucidate the involvement of CeA opioid receptors in the EA effect.Results: High-frequency (100?Hz) EA stimulation of bilateral Feng-Chi acupoints did not suppress the pilocarpine-induced epileptiform electroencephalograms (EEGs), whereas it further increased the duration of epileptiform EEGs. We also observed that epilepsy occurred while 100?Hz EA stimulation of Feng-Chi acupoints was delivered into na?ve rats. EA-induced augmentation of epileptic activity was blocked by microinjection of naloxone, μ- (naloxonazine), κ- (nor-binaltorphimine) or δ-receptor antagonists (natrindole) into the CeA, suggesting that activation of opioid receptors in the CeA mediates EA-exacerbated epilepsy.Conclusions: The present study suggests that high-frequency (100?Hz) EA stimulation of bilateral Feng-Chi acupoints has no effect to protect against pilocarpine-induced focal epilepsy; in contrast, EA further exacerbated focal epilepsy induced by pilocarpine. Opioid receptors in the CeA mediated EA-induced exacerbation of focal epilepsy. ? 2013 Yi et al.; licensee BioMed Central Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/446393 | ISSN: | 1472-6882 | DOI: | 10.1186/1472-6882-13-290 | SDG/關鍵字: | naloxonazine; naloxone; naltrindole; norbinaltorphimine; opiate receptor; pilocarpine; amygdaloid nucleus; animal experiment; animal model; article; cerebellum; controlled study; disease exacerbation; drug response; electroacupuncture; electrode; electroencephalography; epileptic discharge; focal epilepsy; frontal lobe; male; nonhuman; occipital lobe; parietal lobe; rat; right hemisphere; Acupuncture Points; Amygdala; Animals; Electroacupuncture; Epilepsies, Partial; Humans; Male; Narcotic Antagonists; Rats; Rats, Sprague-Dawley; Receptors, Opioid |
顯示於: | 臨床動物醫學研究所 |
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