https://scholars.lib.ntu.edu.tw/handle/123456789/448094
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Zhang, S. | en_US |
dc.contributor.author | Xue, M. | en_US |
dc.contributor.author | Zhang, J. | en_US |
dc.contributor.author | Chen, Q. | en_US |
dc.contributor.author | Chen, J. | en_US |
dc.contributor.author | Wang, Z. | en_US |
dc.contributor.author | Zhou, W. | en_US |
dc.contributor.author | Chen, P. | en_US |
dc.contributor.author | Xia, N. | en_US |
dc.contributor.author | Ge, S. | en_US |
dc.contributor.author | PING-HEI CHEN | en_US |
dc.creator | PING-HEI CHEN;Ge, S.;Xia, N.;Chen, P.;Zhou, W.;Wang, Z.;Chen, J.;Chen, Q.;Zhang, J.;Xue, M.;Zhang, S. | - |
dc.date.accessioned | 2020-01-13T08:23:04Z | - |
dc.date.available | 2020-01-13T08:23:04Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/448094 | - |
dc.description.abstract | Objectives: We have developed a one-step nucleic acid dipstick assay (NADA) for visually detecting polymerase chain reaction (PCR) products within 3. min. "One-step" means that there were no additional procedures between amplification and detection. Methods: This method was achieved through the use of asymmetric PCR and specially designed probes with appropriate melting temperature values. We initially combined one-step NADA with asymmetric capillary convective PCR (ACCPCR), an easy and rapid nucleic acid amplification technique, to construct an on-site nucleic acid diagnostic platform. Results: We developed a diagnostic assay for the hepatitis B virus based on the ACCPCR-NADA platform to verify its feasibility. It exhibited an analytical sensitivity of three copies per test and a broad detection spectrum including genotype A-I. It also showed 97.9% sensitivity and 100% specificity based on the results observed using 67 serum samples with the Roche COBAS AmpliPrep/COBAS TaqMan (COBAS) system as the standard for comparison. Conclusion: The results provide evidence for the feasibility of using an ACCPCR-NADA platform in practical applications, especially in on-site test. ? 2013 The Canadian Society of Clinical Chemists. | - |
dc.relation.ispartof | Clinical Biochemistry | - |
dc.subject | Asymmetric capillary convective PCR; Nucleic acid on-site testing; One-step nucleic acid dipstick assay | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | agar gel electrophoresis; article; controlled study; diagnostic test accuracy study; false negative result; genotype; Hepatitis B virus; human; limit of detection; nonhuman; nucleic acid amplification; nucleic acid analysis; one step dipstick assay; polymerase chain reaction; priority journal; sensitivity and specificity; temperature; virus load; virus strain; 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride; Asymmetric capillary convective PCR; capillary convective PCR; CCPCR; COBAS; EDC; EDTA; ethylenediaminetetraacetic acid; EV71; FITC; fluorescein isothiocyanate; GAM; goat anti-mouse; HBV; HCV; hepatitis B virus; hepatitis C virus; hepatitis E virus; HEV; HIV; HPV; human enterovirus 71; human immunodeficiency virus; human papillomavirus; melting temperature; NADA; National Center for Biotechnology Information; National Institute of Diagnostics and Vaccine Development in Infectious Diseases; NCBI; NIDVD; nt; Nucleic acid on-site testing; nucleotide; one-step nucleic acid dipstick assay; One-step nucleic acid dipstick assay; PCR; polymerase chain reaction; polyvinylchloride; PVC; Roche COBAS AmpliPrep/COBAS TaqMan; SA; streptavidin; TAE; Tm; tris-acetate-EDTA; DNA, Viral; Hepacivirus; Hepatitis B virus; HIV-1; Humans; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction; Sensitivity and Specificity | - |
dc.title | A one-step dipstick assay for the on-site detection of nucleic acid | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.clinbiochem.2013.10.013 | - |
dc.identifier.scopus | 2-s2.0-84889631930 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84889631930&doi=10.1016%2fj.clinbiochem.2013.10.013&partnerID=40&md5=9cfdc76b38b8b58c8c8abc72ff4c3a94 | - |
dc.relation.pages | 1852-1856 | - |
dc.relation.journalvolume | 46 | - |
dc.relation.journalissue | 18 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Mechanical Engineering | - |
crisitem.author.orcid | 0000-0002-8073-9344 | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 機械工程學系 |
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