https://scholars.lib.ntu.edu.tw/handle/123456789/452188
標題: | Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors | 作者: | Lin, C.-Y. Liu, T.-M. Chen, C.-Y. Huang, Y.-L. Huang, W.-K. Sun, C.-K. FU-HSIUNG CHANG CHI-KUANG SUN |
公開日期: | 2010 | 卷: | 146 | 期: | 3 | 起(迄)頁: | 291-298 | 來源出版物: | Journal of Controlled Release | 摘要: | Ultrasound-mediated microbubble destruction may enhance the release of nanoparticles from vasculature to tumor tissues. In this study, we used four different sizes of lipid-coated CdSe quantum dot (LQD) nanoparticles ranging from 30 to 180. nm, 1.0-MHz pulsed focused ultrasound (FUS) with a peak acoustic pressure of 1.2-MPa, and an ultrasound contrast agent (UCA; SonoVue?) at a dose of 30μL/kg to investigate any enhancement of targeted delivery. Tumor-bearing male Balb/c mice were first injected with UCA intravenously, were then sonicated at the tumors with FUS, and were finally injected with 50μL of the LQD solution after the sonication. The mice were sacrificed about 24. h after the sonication, and then we quantitatively and qualitatively evaluated the deposition of LQDs in the tumors by using graphite furnace atomic absorption spectrometry (GF-AAS), photoluminescence spectrometry (PL), and harmonic generation microscopy (HGM). Further, immunoblotting analysis served to identify the biochemical markers reflecting the vascular rupture. The experimental results show that the amount of LQDs deposited in tumor tissues was greater in cases of FUS/UCA application, especially for smaller LQDs, being 4.47, 2.27, 0.99, and 0.82 (μg Cd)/(g tumor) for 30, 80, 130, and 180. nm of LQDs, respectively; compared to 1.12, 0.75, 0.26, and 0.34 (μg Cd)/(g tumor) in absence of FUS/UCA. The immunoblotting analysis further indicates that FUS-induced UCA oscillation/destruction results in rupture areas in blood vessels increasing the vascular permeability and thus justifying for the higher quantity of nanoparticles deposited in tumors. ? 2010 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956285915&doi=10.1016%2fj.jconrel.2010.05.033&partnerID=40&md5=72ae91be53a48df78c54451a419b6101 https://scholars.lib.ntu.edu.tw/handle/123456789/452188 |
ISSN: | 01683659 | DOI: | 10.1016/j.jconrel.2010.05.033 | SDG/關鍵字: | Acoustic pressures; BALB/c mice; Biochemical markers; Different sizes; Focused ultrasound; Graphite furnace atomic absorption spectrometry; Harmonic generation microscopy; Immunoblotting analysis; Micro-bubble; Microbubbles; Mouse tumors; Quantum Dot; Rupture areas; Targeted delivery; Triggered release; Tumor tissues; Ultrasound contrast agent; Vascular permeability; Vasculature; Absorption spectroscopy; Atomic absorption spectrometry; Biochemistry; Blood vessels; Cadmium compounds; Histology; Nanoparticles; Optical waveguides; Semiconductor quantum dots; Ultrasonic transmission; Ultrasonics; Tumors; cadmium selenide; dioleoylphosphatidylethanolamine; lipid; lipid coated cadmium selenide quantum dot; macrogol 2000; nanoparticle; quantum dot; sonovue; unclassified drug; animal cell; animal experiment; animal model; article; Bagg albino mouse; cancer cell culture; controlled study; drug release; male; microbubble; mouse; nonhuman; priority journal; qualitative analysis; quantitative analysis; tumor vascularization; ultrasound; Animals; Blood Vessels; Cadmium Compounds; Drug Delivery Systems; Lipids; Male; Mice; Mice, Inbred BALB C; Microbubbles; Nanoparticles; Neoplasms; P-Selectin; Phospholipids; Quantum Dots; Selenium Compounds; Sonication; Sulfur Hexafluoride; Ultrasonics |
顯示於: | 生物化學暨分子生物學科研究所 |
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