https://scholars.lib.ntu.edu.tw/handle/123456789/452194
Title: | Cell tracking and detection of molecular expression in live cells using lipid-enclosed CdSe quantum dots as contrast agents for epi-third harmonic generation microscopy | Authors: | Chang C.-F. Chen C.-Y. FU-HSIUNG CHANG Tai S.-P. Chen C.-Y. Yu C.-H. Tseng Y.-B. Tsai T.-H. Liu I.-S. Su W.-F. Sun C.-K. |
Issue Date: | 2008 | Publisher: | Optical Society of America (OSA) | Journal Volume: | 16 | Journal Issue: | 13 | Start page/Pages: | 9534-9548 | Source: | Optics Express | Abstract: | We demonstrated that lipid-enclosed CdSe quantum dots (LEQDs) can function as versatile contrast agents in epi-detection third harmonic generation (THG) microscopy for biological applications in vivo. With epi-THG intensities 20 times stronger than corresponding fluorescence intensities from the same LEQDs under the same conditions of energy absorption, such high brightness LEQDs were proved for the abilities of cell tracking and detection of specific molecular expression in live cancer cells. Using nude mice as an animal model, the distribution of LEQD-loaded tumor cells deep in subcutaneous tissues were imaged with high THG contrast. This is the first demonstration that THG contrast can be manipulated in vivo with nanoparticles. By linking LEQDs with anti-Her2 antibodies, the expression of Her2/neu receptors in live breast cancer cells could also be easily detected through THG. Compared with fluorescence modalities, the THG modality also provides the advantage of no photobleaching and photoblinking effects. Combined with a high penetration 1230 nm laser, these novel features make LEQDs excellent THG contrast agents for in vivo deep-tissue imaging in the future. ? 2008 Optical Society of America. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-46149086788&doi=10.1364%2fOE.16.009534&partnerID=40&md5=cde640c61e59276a4850f0d47830545c https://scholars.lib.ntu.edu.tw/handle/123456789/452194 |
ISSN: | 10944087 | DOI: | 10.1364/OE.16.009534 | SDG/Keyword: | Cells; Cytology; Diseases; Fluorescence; Nanocrystals; Photobleaching; Semiconductor quantum dots; Tissue; Biological applications; Breast cancer cells; CdSe quantum dots; Deep tissue imaging; Fluorescence intensities; Live cancer cells; Subcutaneous tissues; Third-harmonic generation microscopy; Harmonic generation; biomaterial; cadmium derivative; cadmium selenide; contrast medium; diagnostic agent; drug carrier; lipid; quantum dot; selenium derivative; animal; article; chemistry; fluorescence microscopy; gene expression profiling; genetic procedures; human; image enhancement; methodology; mouse; neoplasm; nude mouse; pathology; tumor cell line; Animals; Cadmium Compounds; Cell Line, Tumor; Coated Materials, Biocompatible; Contrast Media; Drug Carriers; Gene Expression Profiling; Humans; Image Enhancement; Lipids; Mice; Mice, Nude; Microscopy, Fluorescence; Molecular Probe Techniques; Neoplasms; Quantum Dots; Selenium Compounds |
Appears in Collections: | 生物化學暨分子生物學科研究所 |
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