https://scholars.lib.ntu.edu.tw/handle/123456789/452494
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tsai S.-R. | en_US |
dc.contributor.author | Yin R. | en_US |
dc.contributor.author | Huang Y.-Y. | en_US |
dc.contributor.author | BOR-CHING SHEU | en_US |
dc.contributor.author | SI-CHEN LEE | en_US |
dc.contributor.author | Hamblin M.R. | en_US |
dc.creator | Tsai S.-R.;Yin R.;Huang Y.-Y.;Bor-Ching Sheu;Lee S.-C.;Hamblin M.R. | - |
dc.date.accessioned | 2020-01-22T06:00:13Z | - |
dc.date.available | 2020-01-22T06:00:13Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924770941&doi=10.1016%2fj.pdpdt.2014.10.009&partnerID=40&md5=adf3e7340262b3ed709e79916e3a9f3b | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/452494 | - |
dc.description.abstract | Background: Low-level light therapy (LLLT) is used to stimulate healing, reduce pain and inflammation, and preserve tissue from dying. LLLT has been shown to protect cells in culture from dying after various cytotoxic insults, and LLLT is known to increase the cellular ATP content. Previous studies have demonstrated that maintaining a sufficiently high ATP level is necessary for the efficient induction and execution of apoptosis steps after photodynamic therapy (PDT). Methods: We asked whether LLLT would protect cells from cytotoxicity due to PDT, or conversely whether LLLT would enhance the efficacy of PDT mediated by mono-l-aspartyl chlorin(e6) (NPe6). Increased ATP could lead to enhanced cell uptake of NPe6 by the energy dependent process of endocytosis, and also to more efficient apoptosis. In this study, human osteosarcoma cell line MG-63 was subjected to 1.5J/cm2 of 810nm near infrared radiation (NIR) followed by addition of 10μM NPe6 and after 2h incubation by 1.5J/cm2 of 652nm red light for PDT. Results: PDT combined with LLLT led to higher cell death and increased intracellular reactive oxygen species compared to PDT alone. The uptake of NPe6 was moderately increased by LLLT, and cellular ATP was increased. The mitochondrial respiratory chain inhibitor antimycin A abrogated the LLLT-induced increase in cytotoxicity. Conclusions: Taken together, these results demonstrate that LLLT potentiates NPe6-mediated PDT via increased ATP synthesis and is a potentially promising strategy that could be applied in clinical PDT. ? 2014 Elsevier B.V. | - |
dc.relation.ispartof | Photodiagnosis and Photodynamic Therapy | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | adenosine triphosphate; antimycin A1; aspartic acid derivative; mono levo aspartyl chlorin(e6); reactive oxygen metabolite; unclassified drug; adenosine triphosphate; photosensitizing agent; porphyrin; talaporfin; apoptosis; Article; cell death; cell protection; cell survival; cell viability; cellular distribution; controlled study; cytotoxicity; diode laser; endocytosis; human; human cell; incubation time; infrared radiation; low level laser therapy; low level light therapy; lysosome; mitochondrial respiration; mitochondrion; osteosarcoma cell line; photodynamic therapy; phototherapy; priority journal; red light; cell survival; drug effects; low level laser therapy; metabolism; multimodality cancer therapy; osteosarcoma; pathology; photochemotherapy; procedures; radiation response; treatment outcome; tumor cell line; Adenosine Triphosphate; Apoptosis; Cell Line, Tumor; Cell Survival; Combined Modality Therapy; Humans; Low-Level Light Therapy; Osteosarcoma; Photochemotherapy; Photosensitizing Agents; Porphyrins; Treatment Outcome | - |
dc.title | Low-level light therapy potentiates NPe6-mediated photodynamic therapy in a human osteosarcoma cell line via increased ATP | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.pdpdt.2014.10.009 | - |
dc.identifier.scopus | 2-s2.0-84924770941 | - |
dc.relation.pages | 123-130 | - |
dc.relation.journalvolume | 12 | - |
dc.relation.journalissue | 1 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Photonics and Optoelectronics | - |
crisitem.author.dept | Electronics Engineering | - |
crisitem.author.dept | TSMC-NTU Joint Research Center | - |
crisitem.author.orcid | 0000-0003-1278-6082 | - |
crisitem.author.orcid | 0000-0002-3788-2030 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
Appears in Collections: | 醫學系 |
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