https://scholars.lib.ntu.edu.tw/handle/123456789/452653
標題: | Suppression of B3GNT7 gene expression in colon adenocarcinoma and its potential effect in the metastasis of colon cancer cells | 作者: | Lu, Chun-Hao Wu, Wan-Yi Lai, Yin-Ju Yang, Chen-Ming LUNG-CHIH YU |
關鍵字: | B3GNT7; colon cancer; DNA methylation; metastasis | 公開日期: | 2014 | 卷: | 24 | 期: | 4 | 起(迄)頁: | 359 - 367 | 來源出版物: | Glycobiology | 摘要: | The cell surface sialyl Lewis a (sLea) and sialyl Lewis x (sLex) antigens, which are built on the terminals of glyco-structures called poly-N-acetyllactosamine (LacNAc) chains, have been shown to play a critical role in the metastasis of colon cancer. In the present investigation, expression of the B3GNT7 gene, which encodes a β-1,3-N- acetylglucosaminyltransferase that mainly acts on and extends sulfated poly-LacNAc chains, was found to be markedly suppressed during the oncogenetic processes associated with colon cancer. DNA methylation in the promoter region of the B3GNT7 gene was found to play a significant role in the suppression of the B3GNT7 gene in colon cancer cells. The results obtained from Transwell experiments and the nude mice xenograft model demonstrated that ectopic expression of the B3GNT7 gene in colon cancer cells diminished the migration capability and the liver-metastasis potential, respectively, of colon cancer cells. Flow cytometric analysis showed that expression of cell surface sLe a and sLex antigens was decreased in colon cancer cells when the B3GNT7 gene was ectopically expressed. Taken together, the results of the present investigation suggest a link between suppression of B3GNT7 gene expression and elevation of sLea/sLex antigen expressions on the surface of cells and that this consequently promotes the metastasis potential of cancer cells as part of the colon cancer oncogenetic process. ? The Author 2014. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/452653 https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84898958527&doi=10.1093%2fglycob%2fcwu002&partnerID=40&md5=763e3a72d96084ca15dc244bc0039ceb |
ISSN: | 09596658 | DOI: | 10.1093/glycob/cwu002 | SDG/關鍵字: | membrane antigen; sialyl Lewis a antigen; sialyl Lewis x antigen; unclassified drug; B3GNT1 protein, human; B3GNT7 protein, human; messenger RNA; n acetylglucosaminyltransferase; animal experiment; animal model; article; beta 1,3 n acetylglucosaminyltransferase gene; cancer cell culture; cell migration; cell surface; colon adenocarcinoma; colon cancer; colon carcinogenesis; controlled study; DNA methylation; down regulation; flow cytometry; gene; gene expression; gene repression; human; human cell; human tissue; liver metastasis; male; metastasis; methylation; mouse; nonhuman; priority journal; promoter region; adenocarcinoma; animal; biosynthesis; cell motion; colon tumor; down regulation; gene expression profiling; gene expression regulation; genetics; HCT116 cell line; HT 29 cell line; liver tumor; metabolism; metastasis; nude mouse; pathology; secondary; tumor cell line; Adenocarcinoma; Animals; Cell Line, Tumor; Cell Movement; Colonic Neoplasms; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; HCT116 Cells; HT29 Cells; Humans; Liver Neoplasms; Mice; Mice, Nude; N-Acetylglucosaminyltransferases; Neoplasm Metastasis; RNA, Messenger |
顯示於: | 生化科學研究所 |
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