https://scholars.lib.ntu.edu.tw/handle/123456789/452914
Title: | PAX1/SOX1 DNA methylation and cervical neoplasia detection: A Taiwanese Gynecologic Oncology Group (TGOG) study | Authors: | Lai H.-C. Ou Y.-C. Chen T.-C. Huang H.-J. Cheng Y.-M. CHI-HAU CHEN Chu T.-Y. Hsu S.-T. Liu C.-B. Hung Y.-C. Wen K.-C. Yu M.-H. Wang K.-L. |
Issue Date: | 2014 | Journal Volume: | 3 | Journal Issue: | 4 | Start page/Pages: | 1062-1074 | Source: | Cancer Medicine | Abstract: | We aimed to determine whether PAX1/SOX1 methylation could be translated to clinical practice for cervical neoplasia detection when used alone and in combination with current cytology-based Pap screening. We conducted a multicenter case-control study in 11 medical centers in Taiwan from December 2009 to November 2010. Six hundred seventy-six patients were included in the analysis, including 330 in the training set and 346 in the testing set. Multiplex quantitative methylation-specific polymerase chain reaction (PCR) was performed with a TaqMan probe system using a LightCycler 480 Real-Time PCR System (Roche). The level of human papilloma virus (HPV) was analyzed using a Hybrid Capture 2 system (Digene). Receiver operating characteristic curves were generated to obtain the best cutoff values from the training data set. The sensitivities, specificities, and accuracies were validated in the testing set. The sensitivities for methylated (m) PAX1m and SOX1m and HPV testing for detecting CIN3+ lesions were 0.64, 0.71, and 0.89, and the specificities were 0.91, 0.77, and 0.68, respectively. Combined parallel testing of PAX1m/SOX1m tests with Pap smearing showed superior specificity (0.84/0.71 vs. 0.66, respectively) and similar sensitivity (0.93/0.96 vs. 0.97) to the combination of Pap smear results and HPV testing. Thus, combined parallel testing using Pap smears and PAX1 or SOX1 methylation tests may provide better performance than a combination of Pap smears with HPV testing in detection for cervical neoplasia. ? 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/452914 | DOI: | 10.1002/cam4.253 | SDG/Keyword: | genomic DNA; transcription factor; transcription factor Pax1; transcription factor Sox1; unclassified drug; paired box transcription factor; PAX1 transcription factor; SOX1 protein, human; transcription factor Sox; tumor marker; adult; analytical equipment; article; case control study; controlled study; diagnostic accuracy; diagnostic test accuracy study; DNA methylation; ethnic group; female; histopathology; human; human tissue; major clinical study; molecular probe; multicenter study; multiplex polymerase chain reaction; Papanicolaou test; polymerase chain reaction system; priority journal; receiver operating characteristic; reference value; sensitivity and specificity; Taiwan; Taiwanese; uterine cervix cancer; uterine cervix carcinoma in situ; validation process; virus detection; virus load; Wart virus; aged; Cervical Intraepithelial Neoplasia; clinical trial; genetics; middle aged; Uterine Cervical Neoplasms; very elderly; young adult; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Case-Control Studies; Cervical Intraepithelial Neoplasia; DNA Methylation; Female; Humans; Middle Aged; Paired Box Transcription Factors; ROC Curve; SOXB1 Transcription Factors; Taiwan; Uterine Cervical Neoplasms; Young Adult [SDGs]SDG3 |
Appears in Collections: | 醫學系 |
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