https://scholars.lib.ntu.edu.tw/handle/123456789/452957
標題: | Isoform expression of Na+-K+-ATPase alpha-subunit in gills of the teleost Oreochromis mossambicus | 作者: | Lee, Tsung Han Tsai, Jong Chang Fang, Mei Jane MING-JIUN YU Hwang, Pung Pung |
關鍵字: | Chloride cell | Ion regulation | Sodium pump | 公開日期: | 7-十月-1998 | 卷: | 275 | 期: | 3 44-3 | 來源出版物: | American Journal of Physiology - Regulatory Integrative and Comparative Physiology | 摘要: | Three isoform-specific antibodies, 6F against the α1-isoform of the arian sodium pump, HERED against the rat α2-isoform, and Ax2 against the rat α3-isoform, were used to detect the expression of Na+-K+-ATPase α- subunits in gills of a teleost, the tilapia (Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for α1- and α3-isoforms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of α1-like isoform alone and consequently the ratio of α1-like to α3-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for α1- and α3- isoforms. α1-like and α3-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/452957 | ISSN: | 03636119 | DOI: | 10.1152/ajpregu.1998.275.3.R926 |
顯示於: | 生物化學暨分子生物學科研究所 |
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