https://scholars.lib.ntu.edu.tw/handle/123456789/453065
標題: | Fetal gender determination using real-time quantitative polymerase chain reaction analysis of maternal plasma | 作者: | HSIAO-LIN HWA TSANG-MING KO MEN-LUH YEN Chiang Y.-L. |
公開日期: | 2004 | 卷: | 103 | 期: | 5 | 起(迄)頁: | 364-368 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background and Purpose: Cell-free fetal DNA in maternal blood is a promising non-invasive alternative for prenatal diagnosis. The goal of this study was to determine fetal sex with maternal plasma in pregnant women without previous male offspring. Methods: Blood samples (3 ml) were collected at 6 to 16 weeks' gestation from 56 healthy pregnant women without previous male offspring. There were 23 pregnancies with male fetuses and 33 pregnancies with female fetuses. DNA was extracted from plasma and qualitative analysis of the sex-determining region Y (SRY) gene sequence on the Y chromosome (SRY-3317T) with dual-labeled fluorescent TaqMan probe using real-time quantitative polymerase chain reaction (PRC) was performed. Results: In the 56 successfully assayed sample, SRY-positive signals were detected in 20 samples of the 23 pregnancies with male fetuses. None of the 33 successfully assayed samples from women bearing female fetuses yielded a positive signal. Conclusion: Because of its high correct prediction rate, fetal gender determination with maternal plasma using real-time quantitative PCR can be used as a pre-test to avoid unneccessary chorionic villus sampling for pregnancies at risk of fetal sex-associated disorder. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/453065 | SDG/關鍵字: | DNA; fluorescent dye; DNA; article; blood analysis; blood sampling; chorion villus sampling; controlled study; diagnostic accuracy; diagnostic value; DNA extraction; female; fetus; gene sequence; gestation period; high risk pregnancy; human; major clinical study; male; maternal plasma; molecular probe; prediction; pregnancy; prenatal diagnosis; progeny; qualitative analysis; real time polymerase chain reaction; sensitivity and specificity; sex determination; sex differentiation disorder; signal detection; SRY gene; Y chromosome; adult; blood; evaluation; genetics; methodology; polymerase chain reaction; Adult; Chromosomes, Human, Y; DNA; Female; Humans; Male; Polymerase Chain Reaction; Pregnancy; Prenatal Diagnosis; Sensitivity and Specificity; Sex Determination (Analysis) |
顯示於: | 法醫學科所 |
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