|Title:||Accumulation of free cholesterol and oxidized low-density lipoprotein is associated with portal inflammation and fibrosis in nonalcoholic fatty liver disease||Authors:||CHENG-MAW HO
|Issue Date:||2019||Publisher:||BioMed Central Ltd.||Journal Volume:||16||Journal Issue:||1||Source:||Journal of Inflammation (United Kingdom)||Abstract:||
Background: Macrophages engulf oxidized-LDL (oxLDL) leading to accumulation of cellular cholesterol and formation of foam cells, which is a hallmark of atherosclerosis. Moreover, recent studies showed that accumulation of free cholesterol in macrophages leading to activation of NLRP3 inflammasome and production of interleukin-1β (IL-1β) has been linked to atherosclerosis-associated inflammation. However, it is not clear if cholesterol accumulation is associated with hepatic inflammation and fibrosis in the liver. In this study, we investigated the association of free cholesterol and oxLDL accumulation in portal vein with the inflammation, atherosclerosis, and fibrosis in human nonalcoholic fatty liver disease (NAFLD). Methods: Serial sections derived from surgical specimens of NAFLD were stained with filipin and antibodies against IL-1β, CD68, α-smooth muscle actin (α-SMA), oxLDL and lectin-like oxLDL receptor-1 (LOX-1). Results: We show that free cholesterol was colocalized with oxLDL in the wall of portal vein, and which was associated with lumen narrowing, plaque formation, endothelium deformation, and portal venous inflammation. The inflammation was evidenced by the colocalization of Kupffer cells and IL-1β and the expression of LOX-1. Notably, ruptured plaque was closely associated with portal venous inflammation. Moreover, free cholesterol and oxLDL accumulation in periportal and sinusoidal fibrosis, which was associated with regional stellate cell activation and chicken-wire fibrosis. Conclusion: These findings reveal a direct association between cholesterol accumulation, portal venous inflammation and fibrosis in NAFLD. ? 2019 The Author(s).
|ISSN:||1476-9255||DOI:||10.1186/s12950-019-0211-5||SDG/Keyword:||alpha smooth muscle actin; antibody; CD68 antigen; cholesterol; filipin; interleukin 1beta; lectin like oxidized low density lipoprotein; oxidized low density lipoprotein; oxidized low density lipoprotein receptor 1; unclassified drug; aged; Article; atherosclerosis; atherosclerotic plaque; cell activation; cellular distribution; chicken wire fibrosis; controlled study; disease association; endothelium deformation; gene overexpression; hepatic portal vein; hepatic stellate cell; hepatitis; human; human cell; human tissue; inflammation; Kupffer cell; liver cirrhosis; liver fibrosis; liver parenchyma; male; nonalcoholic fatty liver; pathogenesis; protein expression; protein localization; sinusoidal fibrosis; staining; very elderly
|Appears in Collections:||生物化學暨分子生物學科研究所|
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