|Title:||Radix Scrophulariae extracts (harpagoside) suppresses hypoxia-induced microglial activation and neurotoxicity||Authors:||Sheu S.-Y.
|Issue Date:||2015||Publisher:||BioMed Central Ltd.||Journal Volume:||15||Journal Issue:||1||Source:||BMC Complementary and Alternative Medicine||Abstract:||
Background: Hypoxia could lead to microglia activation and inflammatory mediators' overproduction. These inflammatory molecules could amplify the neuroinflammatory process and exacerbate neuronal injury. The aim of this study is to find out whether harpagoside could reduce hypoxia-induced microglia activation. Methods: In this study, primary microglia cells harvested from neonatal ICR mice were activated by exposure to hypoxia (1 % O2 for 3 h). Harpagoside had been shown to be no cytotoxicity on microglia cells by MTT assay. The scavenger effect of harpagoside on hypoxia-enhanced microglial cells proliferation, associated inflammatory genes expression (COX-II, IL-1β and IL-6 genes) and NO synthesis were also examined. Results: Hypoxia enhances active proliferation of microglial cells, while harpagoside can scavenge this effect. We find that harpagoside could scavenge hypoxia-enhanced inflammatory genes expression (COX-2, IL-1β and IL-6 genes) and NO synthesis of microglial cells. Under 3 h' hypoxic stimulation, the nuclear contents of p65 and hypoxia inducible factor-1aα (HIF-1aα) significantly increase, while the cytosol IkB-aα content decreases; these effects can be reversed by 1 h's pre-incubation of 10-8 M harpagoside. Harpagoside could decrease IkB-aα protein phosphorylation and inhibit p65 protein translocation from the cytosol to the nucleus, thus suppress NF-kB activation and reduce the HIF-1aα generation. Conclusion: These results suggested that the anti-inflammatory mechanism of harpagoside might be associated with the NF-kB signaling pathway. Harpagoside protect against hypoxia-induced toxicity on microglial cells through HIF-aα pathway. ? 2015 Sheu et al.
|ISSN:||1472-6882||DOI:||10.1186/s12906-015-0842-x||SDG/Keyword:||complementary DNA; cyclooxygenase 2; harpagoside; hypoxia inducible factor 1alpha; I kappa B alpha; immunoglobulin enhancer binding protein; interleukin 1beta; interleukin 6; nitric oxide; RNA; synaptotagmin I; glycoside; harpagoside; plant extract; protective agent; pyran derivative; animal cell; Article; cell activation; cell nucleus; cell proliferation; cell viability; controlled study; cytosol; DNA synthesis; drug effect; exposure; gene expression; hypoxia; microglia; mouse; neurotoxicity; newborn; nonhuman; protein phosphorylation; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; Western blotting; animal; chemistry; drug effects; inflammation; Institute for Cancer Research mouse; metabolism; microglia; Scrophularia; Animals; Gene Expression; Glycosides; Hypoxia; Inflammation; Mice; Mice, Inbred ICR; Microglia; Plant Extracts; Protective Agents; Pyrans; Scrophularia
|Appears in Collections:||醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.