https://scholars.lib.ntu.edu.tw/handle/123456789/455316
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen, C.-Y. | en_US |
dc.contributor.author | Ke, C.-J. | en_US |
dc.contributor.author | Yen, K.-C. | en_US |
dc.contributor.author | Hsieh, H.-C. | en_US |
dc.contributor.author | JUI-SHENG SUN | en_US |
dc.contributor.author | FENG-HUEI LIN | en_US |
dc.creator | Chen C.-Y.;Ke C.-J.;Yen K.-C.;Hsieh H.-C.;Jui-Sheng Sun;Lin F.-H. | - |
dc.date.accessioned | 2020-02-07T03:43:32Z | - |
dc.date.available | 2020-02-07T03:43:32Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1838-7640 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930622801&doi=10.7150%2fthno.11372&partnerID=40&md5=bf3f40770c89023ef36294a4a41206cc | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/455316 | - |
dc.description.abstract | Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A platform consisting of human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, and a self-made bioreactor system was established for autologous transplantation of human osteoblast cell clusters. The Ca-Alginate scaffold facilitated the growth and differentiation of human bone cell clusters, and the functionally-closed process bioreactor system supplied the soluble nutrients and osteogenic signals required to maintain the cell viability. This system preserved the proliferative ability of cells and cell viability and up-regulated bone-related gene expression and biological apatite crystals formation. The bone-like tissue generated could be extracted by removal of calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation, and exhibited a size suitable for injection. The described strategy could be used in therapeutic application and opens new avenues for surgical interventions to correct skeletal defects. ? 2015 Ivyspring International Publisher. | - |
dc.publisher | Ivyspring International Publisher | - |
dc.relation.ispartof | Theranostics | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | alkaline phosphatase; beta 2 microglobulin; calcium alginate; collagen type 1; edetic acid; messenger RNA; osteocalcin; transcription factor RUNX2; alginic acid; glucuronic acid; hexuronic acid; Alp1 gene; Article; autotransplantation; B2M gene; Bglap gene; bioreactor; bone density; bone development; bone mass; cell proliferation; cell transplantation; cell viability; chelation; Col1a1 gene; controlled study; crystallization; culture technique; gene expression regulation; human; human cell; osteoblast; porosity; process development; Runx2 gene; scanning electron microscopy; three dimensional cell culture system; three dimensional imaging; tissue engineering; tissue scaffold; cell culture; chemistry; cytology; devices; drug effects; osteoblast; physiology; procedures; tissue scaffold; Alginates; Bioreactors; Cell Proliferation; Cells, Cultured; Glucuronic Acid; Hexuronic Acids; Humans; Osteoblasts; Tissue Engineering; Tissue Scaffolds | - |
dc.title | 3D porous calcium-alginate scaffolds cell culture system improved human osteoblast cell clusters for cell therapy | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.7150/thno.11372 | - |
dc.identifier.pmid | 25825603 | - |
dc.identifier.scopus | 2-s2.0-84930622801 | - |
dc.relation.pages | 643-655 | - |
dc.relation.journalvolume | 5 | - |
dc.relation.journalissue | 6 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Orthopedic Surgery | - |
crisitem.author.dept | Orthopedic Surgery-NTUH | - |
crisitem.author.dept | Biomedical Engineering | - |
crisitem.author.orcid | 0000-0001-5142-8777 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。